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  Section: General Biotechnology / Genes & Genetic Engineering
 
 
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Techniques of Genetic Engineering

 
     
 
Content

Gene cloning in prokaryotes

 

Isolation of DNA to be cloned 

 

Insertion of DNA fragment into vector 

   

Use of restriction Linkers

   

Use of homopolymer tails

 

Transfer of recombinant DNA into bacterial cells

 

Selection of clones

   

Colony hybridization techniques

   

In vitro translation technique

   

Immunological tests

   

Blotting Techniques

 

Recovery of cells

 

Expression of cloned DNA

   

Shine-Dalgano sequence

   

Expression vectors

Gene cloning in eukaryotes

 

Plant cells

   

Yeasts

   

Filamentous fungi

   

Agrobacterium plasmids

   

Plant cell transformation

   

Plant cell transformation by ultrasonication

   

Liposome mediated gene transfer

 

Animal cell  

   

Animal viruses

   

Electroporation

   

Particle bombardment

   

Microinjection

   

Direct transformation

Site directed mutagenesis

 

Methods of mutagenesis



 
     
 
 
     




     
 
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