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  Section: General Biotechnology / Microbial Biotechnology
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Single Cell Protein (SCP) and Mycoprotein


Cultivation of Dhingri (Pleurotus sajor-caju)

Pleurotus is also one of the important edible mushrooms gaining popularity in recent years. It is found growing naturally on dead organic materials rich in cellulose. Its several species are edible such as P. sajor-caju, P. sapidus, P.flabellatus, P. ostriatus, P. corticatus, P.jlorida, etc. These species can be cultured successfully on various agricultural, domestic, industrial and forestry waste materials. It is very versatile in nature as far as substrate reference and growth are concerned. However, it can be grown on paddy straw, gunny bags, rice husk, copped Parthenium stem, etc. The steps for cultivation of dhingri start with preparation of substrate for growth.


Paddy is cut into 2.5 cm long pieces and soaked in hot water at 60°C for about 30 minutes. Excess water is drained off from straw. About 4 kg of wet straw is transferred into the large sized polythene bags. About 5 grams of bengal-gram powder with half bottle of spawn of fungus are mixed with straw. This mixture is filled in large-sized polythene bags. Mouth of the bags is tied and kept on a raised platform (Fig. 18.10) in well ventilated cropping room or in open when properly protected for about 15 days. At this time when mycelia are visible inside the polythene bags over the surface of paddy straw, the polythene bags are cut and gently removed. Now paddy straw forms a compact mass and does not lose its make up. This composite mixture is watered daily just to maintain moisture. The temperature where the compost has been kept should be between 20°C and 25°C with relative humidity of 75 per cent. After 15 days first flash of dhingri becomes apparent. These are harvested when become young. A photograph of P. sajor-caju growing on paddy straw is shown in Fig. 18.10.

Pleurotus sajor-caju growing on paddy straw
Fig. 18.10. Pleurotus sajor-caju growing on paddy straw


Advantages of producing microbial protein

Microorganisms use as single cell protein (SCP)

Substrates used for the production of SCP

Nutritional values of SCP

Genetic improvements of microbial cells

Production of algal biomass


Factors affecting bio­mass production


Harvesting the algal biomass


Spirulina as SCP, cultivation and uses

Production of bacterial and actinomycetous biomass


Method of production


Factors affecting biomass production


Product recovery

Production of yeast biomass


Factors affecting growth of yeast


Recovery of yeast biomass

Production of fungal biomass (Other than Mushrooms)


Growth conditions


Organic wastes as substrates


Traditional fungal foods













Mushroom culture


Historical background


Present status of mushroom culture in India


Nutritional values


Cultivation methods



Obtaining pure culture 



Preparation of spawns



Formulation and preparation of composts



Spawning, spawn running and cropping


Control of pathogens and pests


Cultivation of paddy straw mushroom


Cultivation of white button mushroom


Cultivation of Dhingri (Pleurotus sajor-caju)


Recipes of mushroom


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