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  Section: General Biotechnology / Plant Biotechnology
 
 
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In Vitro Culture Techniques : The Biotechnological Principles

 
     
 
Mentor Pollen Technology
Pollination is a natural phenomenon which occurs in many plant species. However, there are certain plants where pollen grains remain viable but unable to set seed on its own pistil i.e. they are self incompatible. Similarly, in several other plants, besides being viable they even fail to germinate on stigmas of another species i.e. they are interspecific incompatible.
 

After the birth of recent mentor pollen technology the incompatibility problem of pollen grains can be overcome by altering the fertilization ability. The pollen which has been purposefully treated is called mentor pollen. Hence, the mentor pollen may be defined as the compatible pollen which has been treated in many ways to inhibit its fertilization ability and retain its power to stimulate incompatible pollen to accomplish fertilization. The mentor pollen itself may or may not germinate on stigmas. As a result of this treatment, hybridization between closely related species or genera, and itself pollination in cross-pollinating plants could be achieved. Knox et al. (1987) have reviewed the mentor pollen technology and suggested its benefits as a tool in plant biotechnology. The advantages that it offers are : (i) transfer of specific genes required in a breeding strategy through seeds, (ii) quantification of its effects, and (iii) understanding the pollen-stigma recognition.
 

Content

Totipotency

Historical background

Requirements for cell and Tissue Cultures

 

A tissues culture laboratory

 

Nutrient media

 

 

Inorganic chemicals

 

 

Growth hormones

 

 

Organic constitutents

 

 

Vitamins

 

 

Amino acids

Culture of plant materials

 

Explant culture

 

Callus formation and its culture

 

Organogenesis

 

Root culture

 

Shoot culture and micropropagation

 

Cell culture

 

 

Benefits from cell culture

 

Somatic embryogenesis

 

Somaclonal variation

 

Protoplast culture

 

 

Isolation

 

 

Regeneration

 

Protoplast fusion and somatic hybridization

 

 

Fusion products

 

 

Method of somatic hybridization

 

Anther and pollen Culture

 

 

Culturing techniques

 

In vitro androgenesis (direct and indirect androgenesis)

 

Mentor pollen technology

 

Embryo culture

 

Embryo rescue

 

Protoplast fusion in fungi

 
     
 
 
     



     
 
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