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  Section: Biotechnology Methods » Cell Biology and Genetics
 
 
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Culturing and Observation of Paramecium

 
     
 
Content
Cell Biology and Genetics
  Cell Cycles
  Meiosis in Flower Buds of Allium Cepa-Acetocarmine Stain
  Meiosis in Grasshopper Testis (Poecilocerus Pictus)
  Mitosis in Onion Root Tip (Allium Cepa)
  Differential Staining of Blood
  Buccal Epithelial Smear and Barr Body
  Vital Staining of DNA and RNA in Paramecium
  Induction of Polyploidy
  Mounting of Genitalia in Drosophila Melanogaster
  Mounting of Genitalia in the Silk Moth Bombyx Mori
  Mounting of the Sex Comb in Drosophila Melanogaster
  Mounting of the Mouth Parts of the Mosquito
  Normal Human Karyotyping
  Karyotyping
  Black and White Film Development and Printing for Karyotype Analysis
  Study of Drumsticks in the Neutrophils of Females
  Study of the Malaria Parasite
  Vital Staining of DNA and RNA in Paramecium
  Sex-Linked Inheritance in Drosophila Melanogaster
  Preparation of Somatic Chromosomes from Rat Bone Marrow
  Chromosomal Aberrations
  Study of Phenocopy
  Study of Mendelian Traits
  Estimation of Number of Erythrocytes [RBC] in Human Blood
  Estimation of Number of Leucocytes (WBC) in Human Blood
  Culturing Techniques and Handling of Flies
  Life Cycle of the Mosquito (Culex Pipiens)
  Life Cycle of the Silkworm (Bombyx Mori)
  Vital Staining of Earthworm Ovary
  Culturing and Observation of Paramecium
  Culturing and Staining of E.coli (Gram’s Staining)
  Breeding Experiments in Drosophila Melanogaster
  Preparation of Salivary Gland Chromosomes
  Observation of Mutants in Drosophila Melanogaster
  ABO Blood Grouping and Rh Factor in Humans
  Determination of Blood Group and Rh Factor
  Demonstration of the Law of Independent Assortment
  Demonstration of Law of Segregation

Materials
  • Micropipette
  • Hay stems
  • Wheat
  • Paramecium
Procedure
It is abundantly found in ponds, ditches, and decaying vegetation.
  1. Boil 20 gms of wheat and 20–25 hay stems in 500 cc of distilled water for about 10 minutes.
  2. Keep the culture in a dark and cool place for about 4 days and then inoculate it with a few paramecia with a micropipette.
  3. Within a few days, the culture contains numerous paramecia.
  4. Observe the paramecia under microscope.
Classification
Phylum: Protozoa
Class: Ciliata
Order: Holotricha
Family: Paramecidae
Genus: Paramecium
Species: Caudatum, aurelia

Paramecium is the best-known ciliate, found in fresh water ponds, rivers, lakes, ditches, streams, and pools. It has cosmopolitan distribution. It is commonly called the slipper animalcule. Its anterior end is bluntly rounded, while its posterior end is pointed. Paramecium caudatum measures 80–350 microns. Paramecium aurelia measures 120–290 microns. Cilia cover the entire body. The ventral surface is marked by the presence of an obliquely longitudinal groove, the oral groove or peristome. Reproduction is by binary fission. Conjugation constitutes the sexual part
of the reproduction.
 
     
 
 
     




     
 
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