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  Section: Biotechnology Methods » Cell Biology and Genetics
 
 
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Mounting of Genitalia in the Silk Moth Bombyx Mori

 
     
 
Content
Cell Biology and Genetics
  Cell Cycles
  Meiosis in Flower Buds of Allium Cepa-Acetocarmine Stain
  Meiosis in Grasshopper Testis (Poecilocerus Pictus)
  Mitosis in Onion Root Tip (Allium Cepa)
  Differential Staining of Blood
  Buccal Epithelial Smear and Barr Body
  Vital Staining of DNA and RNA in Paramecium
  Induction of Polyploidy
  Mounting of Genitalia in Drosophila Melanogaster
  Mounting of Genitalia in the Silk Moth Bombyx Mori
  Mounting of the Sex Comb in Drosophila Melanogaster
  Mounting of the Mouth Parts of the Mosquito
  Normal Human Karyotyping
  Karyotyping
  Black and White Film Development and Printing for Karyotype Analysis
  Study of Drumsticks in the Neutrophils of Females
  Study of the Malaria Parasite
  Vital Staining of DNA and RNA in Paramecium
  Sex-Linked Inheritance in Drosophila Melanogaster
  Preparation of Somatic Chromosomes from Rat Bone Marrow
  Chromosomal Aberrations
  Study of Phenocopy
  Study of Mendelian Traits
  Estimation of Number of Erythrocytes [RBC] in Human Blood
  Estimation of Number of Leucocytes (WBC) in Human Blood
  Culturing Techniques and Handling of Flies
  Life Cycle of the Mosquito (Culex Pipiens)
  Life Cycle of the Silkworm (Bombyx Mori)
  Vital Staining of Earthworm Ovary
  Culturing and Observation of Paramecium
  Culturing and Staining of E.coli (Gram’s Staining)
  Breeding Experiments in Drosophila Melanogaster
  Preparation of Salivary Gland Chromosomes
  Observation of Mutants in Drosophila Melanogaster
  ABO Blood Grouping and Rh Factor in Humans
  Determination of Blood Group and Rh Factor
  Demonstration of the Law of Independent Assortment
  Demonstration of Law of Segregation

Description
Genital structures in silk moths are very important for copulation and transferring the gametes. Male genitalia is called the herpes and the female genitalia is called the labius. Herpes is present at the terminal part of the abdomen of the male, and has 2 strong hooks to hold the female labius during copulation. Labius are present at the terminal part of the body which are fleshy with sensory hairs, which help in copulation and transferring the gametes.

Materials
  • Live male and female moths
  • Dissection set
  • Needles
  • Blades
  • Slides
Procedure
  1. Remove the last segment of the abdomen with the help of a blade or needle under the dissection microscope.
  2. Keep the dissected segment in 10% KOH for 5 minutes.
  3. Clean the tissue of the segment.
  4. Observe the labius or herpes under the microscope.
Observation
Both herpes and labius were observed.

 
     
 
 
     




     
 
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