Study of drumsticks in neutrophils of females.
The sex chromatin of polymorphic nuclear neutrophils of human blood contains
a specific “drumstick”-like nuclear appendages that has its head about 1.5 mm
in diameter attached to the nucleus by a threadlike stalk. The drumstick differs
from the sex chromatin of other cells by being extruded from the nucleus. It is
visible in only a relatively small portion of cells (in about 1/40 neutrophils of
a normal female).
- Female blood sample
- Cover slip
- Leishmann stain
- Clean the fingertip with cotton soaked in alcohol and prick it with a
- Place 1 or 2 drops of blood on the right side of the slide.
- With the help of another clean slide, smear the blood along the slide, such
that a tongue-shaped thin-layered smear was formed and air-dried.
- Fix the dried smear with acetone-free methanol or absolute alcohol for
about 5 minutes.
- Dry and stain the smear with Leishmann stain.
- Add distilled water, about double the amount of the stain, on the smear.
- Mix the smear using a pipette for 10–20 minutes.
- Keep the slide in running water to remove excess stain, and then air-dry.
- Observe the slide under the microscope using an oil immersion lens.