Study of Drumsticks in the Neutrophils of Females


Cell Biology and Genetics
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  Mitosis in Onion Root Tip (Allium Cepa)
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  Mounting of the Sex Comb in Drosophila Melanogaster
  Mounting of the Mouth Parts of the Mosquito
  Normal Human Karyotyping
  Black and White Film Development and Printing for Karyotype Analysis
  Study of Drumsticks in the Neutrophils of Females
  Study of the Malaria Parasite
  Vital Staining of DNA and RNA in Paramecium
  Sex-Linked Inheritance in Drosophila Melanogaster
  Preparation of Somatic Chromosomes from Rat Bone Marrow
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  Study of Mendelian Traits
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  Estimation of Number of Leucocytes (WBC) in Human Blood
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  Observation of Mutants in Drosophila Melanogaster
  ABO Blood Grouping and Rh Factor in Humans
  Determination of Blood Group and Rh Factor
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Study of drumsticks in neutrophils of females.

The sex chromatin of polymorphic nuclear neutrophils of human blood contains a specific “drumstick”-like nuclear appendages that has its head about 1.5 mm in diameter attached to the nucleus by a threadlike stalk. The drumstick differs from the sex chromatin of other cells by being extruded from the nucleus. It is visible in only a relatively small portion of cells (in about 1/40 neutrophils of a normal female).

  • Female blood sample
  • Slides
  • Needles
  • Cover slip
  • Alcohol
  • Cotton
  • Leishmann stain
  • Microscope
  1. Clean the fingertip with cotton soaked in alcohol and prick it with a sterilized needle.
  2. Place 1 or 2 drops of blood on the right side of the slide.
  3. With the help of another clean slide, smear the blood along the slide, such that a tongue-shaped thin-layered smear was formed and air-dried.
  4. Fix the dried smear with acetone-free methanol or absolute alcohol for about 5 minutes.
  5. Dry and stain the smear with Leishmann stain.
  6. Add distilled water, about double the amount of the stain, on the smear.
  7. Mix the smear using a pipette for 10–20 minutes.
  8. Keep the slide in running water to remove excess stain, and then air-dry.
  9. Observe the slide under the microscope using an oil immersion lens.