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  Section: Biotechnology Methods » Cell Biology and Genetics
 
 
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Vital Staining of DNA and RNA in Paramecium

 
     
 
Content
Cell Biology and Genetics
  Cell Cycles
  Meiosis in Flower Buds of Allium Cepa-Acetocarmine Stain
  Meiosis in Grasshopper Testis (Poecilocerus Pictus)
  Mitosis in Onion Root Tip (Allium Cepa)
  Differential Staining of Blood
  Buccal Epithelial Smear and Barr Body
  Vital Staining of DNA and RNA in Paramecium
  Induction of Polyploidy
  Mounting of Genitalia in Drosophila Melanogaster
  Mounting of Genitalia in the Silk Moth Bombyx Mori
  Mounting of the Sex Comb in Drosophila Melanogaster
  Mounting of the Mouth Parts of the Mosquito
  Normal Human Karyotyping
  Karyotyping
  Black and White Film Development and Printing for Karyotype Analysis
  Study of Drumsticks in the Neutrophils of Females
  Study of the Malaria Parasite
  Vital Staining of DNA and RNA in Paramecium
  Sex-Linked Inheritance in Drosophila Melanogaster
  Preparation of Somatic Chromosomes from Rat Bone Marrow
  Chromosomal Aberrations
  Study of Phenocopy
  Study of Mendelian Traits
  Estimation of Number of Erythrocytes [RBC] in Human Blood
  Estimation of Number of Leucocytes (WBC) in Human Blood
  Culturing Techniques and Handling of Flies
  Life Cycle of the Mosquito (Culex Pipiens)
  Life Cycle of the Silkworm (Bombyx Mori)
  Vital Staining of Earthworm Ovary
  Culturing and Observation of Paramecium
  Culturing and Staining of E.coli (Gram’s Staining)
  Breeding Experiments in Drosophila Melanogaster
  Preparation of Salivary Gland Chromosomes
  Observation of Mutants in Drosophila Melanogaster
  ABO Blood Grouping and Rh Factor in Humans
  Determination of Blood Group and Rh Factor
  Demonstration of the Law of Independent Assortment
  Demonstration of Law of Segregation

Description
DNA and RNA are 2 types of nucleic acids that have different staining properties. DNA is acidic and stains acidic dyes, while RNA, along with proteins, stains basic dyes. The 2 stains used are methyl green and pyronine. DNA stains blue or bluish-green with methyl green, and RNA stains pink with pyronine dyes. DNA is present in the nucleus and stains blue or bluish-green, while RNA is present in the cytoplasm and stains pink.


Materials
  • Cultured paramecia
  • Cavity slides
  • Plain slides
  • Cover slips
  • Methyl green and pyronine stains 0.5%
Procedure
  1. Pipette out a few paramecia onto a cavity slide.
  2. Blot out excess water using filter paper.
  3. Put 2 or 3 drops of methyl green pyronine stain and keep it for 5 to 10
    minutes.
  4. Transfer the paramecia onto a clean slide. Put them under a cover slip in an aqueous medium and observe under the microscope.

Observation
DNA (nucleus) stain blue and RNA (cytoplasm) stain pink.

 
     
 
 
     




     
 
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