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  Section: Biotechnology Methods » Cell Biology and Genetics
 
 
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Vital Staining of Earthworm Ovary

 
     
 
Content
Cell Biology and Genetics
  Cell Cycles
  Meiosis in Flower Buds of Allium Cepa-Acetocarmine Stain
  Meiosis in Grasshopper Testis (Poecilocerus Pictus)
  Mitosis in Onion Root Tip (Allium Cepa)
  Differential Staining of Blood
  Buccal Epithelial Smear and Barr Body
  Vital Staining of DNA and RNA in Paramecium
  Induction of Polyploidy
  Mounting of Genitalia in Drosophila Melanogaster
  Mounting of Genitalia in the Silk Moth Bombyx Mori
  Mounting of the Sex Comb in Drosophila Melanogaster
  Mounting of the Mouth Parts of the Mosquito
  Normal Human Karyotyping
  Karyotyping
  Black and White Film Development and Printing for Karyotype Analysis
  Study of Drumsticks in the Neutrophils of Females
  Study of the Malaria Parasite
  Vital Staining of DNA and RNA in Paramecium
  Sex-Linked Inheritance in Drosophila Melanogaster
  Preparation of Somatic Chromosomes from Rat Bone Marrow
  Chromosomal Aberrations
  Study of Phenocopy
  Study of Mendelian Traits
  Estimation of Number of Erythrocytes [RBC] in Human Blood
  Estimation of Number of Leucocytes (WBC) in Human Blood
  Culturing Techniques and Handling of Flies
  Life Cycle of the Mosquito (Culex Pipiens)
  Life Cycle of the Silkworm (Bombyx Mori)
  Vital Staining of Earthworm Ovary
  Culturing and Observation of Paramecium
  Culturing and Staining of E.coli (Gram’s Staining)
  Breeding Experiments in Drosophila Melanogaster
  Preparation of Salivary Gland Chromosomes
  Observation of Mutants in Drosophila Melanogaster
  ABO Blood Grouping and Rh Factor in Humans
  Determination of Blood Group and Rh Factor
  Demonstration of the Law of Independent Assortment
  Demonstration of Law of Segregation

To mount and stain earthworm ovary using Janus green stain.

Materials
  • Well-grown earthworm
  • Dissection set
  • Janus green stain
  • Clean glass slide
Procedure
The earthworm is a hermaphrodite. A pair of ovaries is situated in its thirteenth segment. They are pyriform, semi-transparent, hanging freely into the coelom, and attached by their broad ends to the septum of the twelfth and thirteenth segments. Each ovary is a white, compact mass made up of finger-like processes. Each ovary contains ova in a linear series. Each ovum is large, with a distinct nucleus and nucleolus.

Take a full-size earthworm and open it dorsally. Cut the intestine, invert it, and ventrally below the heart the ovaries are seen as white dots. Pick them up carefully, with the help of a forcep, and place them on the glass slides. Use 2 drops of Janus green stain. Leave it for 5 minutes and observe under the microscope.

 
     
 
 
     




     
 
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