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  Section: Biotechnology Methods » Tests
 
 
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Oxidase Test

 
     
 
Content
Hydrolysis/Catalase/Oxidase Tests
  Starch Hydrolysis Test
  Gelatin Hydrolysis Test
  Catalase Test
  Oxidase Test

Aim
To test the oxidase-producing microorganisms.

Principle
The oxidase determines whether microbes can oxidize certain aromatic amines, e.g., paraaminodimethylalanine, to form colored end products. This oxidation correlates with the cytochrome oxidase activity of some bacteria, including the genera Pseudomonas and Nisseria. A positive test is important in identifying these genera, and also useful in characterizing the Enterobacteria, which are oxidase negative.

Materials
  • Glassware
  • Sample
  • Culture of Pseudomonas
  • Bacillus
  • E.coli.
  • Staphylococcus aureus
  • Klebsiella
Chemicals
  • Tetramethyl phenyl diamine
  • Dihydrochloride
Procedure
  1. Plate method: Separate agar plates streaked with Pseudomonas, Klebsiella, and Bacillus are taken, 1% reagent tetra methyl phenyl diamine hydrochloride was directly added to the plates, and the reaction were observed.
Result
EMB
FIGURE 1 EMB.
 
Lactose broth
FIGURE 2 Lactose broth.

Mac conkey
FIGURE 3 Mac conkey.
 
Urease
FIGURE 4 Urease.


 
     
 
 
     




     
 
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