Mechanism of chromosome transfer

Sexuality and Recombination in Bacteria and Viruses
Three Methods for Transfer of Genetic Material 
Sexual conjugation in bacteria 
Culture media and mutant strains
Discovery of gene transfer
Discovery of linkage in bacteria
Donor and recipient strains
- Mechanism of chromosome transfer
- Physical structures involved in chromosome transfer
Linkage maps in bacteria
Conjugation mapping through interrupted mapping
Circular linkage map
Linkage information from transformation
Recombination after gene transfer
High resolution mapping
Linear order of genes
Replication and recombination in viruses 
Replication of bacteriophages
Lysogenic bacteria
Recombination in viruses
Circular genetic maps in viruses

If the Hfr or F+ donates its chromosome to F- during conjugation, the donor should actually die after conjugation. But this does not happen. There is evidence that Hfr chromosome replicates shortly before or during conjugation and only one of the two strands is transferred to F- cell and gets replicated there. This leaves a complete chromosome in Hfr or F+ even after conjugation.