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  Section: Microbiology Methods » Diagnostic Microbiology In Action
 
 
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Identification of an Unknown Enteric Organism

 
     
 
Content
Diagnostic Microbiology In Action
  Microbiology of the Intestinal Tract
    The Enterobacteriaceae (Enteric Bacilli)
      Identification of Pure Cultures of Enterobacteriaceae from the Normal Intestinal Flora
      Isolation Techniques for Enteric Pathogens
      Identification Techniques for Enteric Pathogens
      Serological Identification of Enteric Organisms
      Techniques to Distinguish Nonfermentative Gram-Negative Bacilli from Enterobacteriaceae
      Rapid Methods for Bacterial Identification
    Clinical Specimens from the Intestinal Tract
      Culturing a Fecal Sample
      Identification of an Unknown Enteric Organism
      Antimicrobial Susceptibility Test of an Enteric Organism



Purpose To use the techniques you have learned to identify an unknown pure culture
Materials Same as in Experiments 24.2 and 24.3 except that your assigned culture is numbered, not labeled


Procedures
  1. Prepare a Gram stain of your culture.
  2. Inoculate the culture on EMB or MacConkey, HE, and BiS plates, and streak for isolation.
  3. Inoculate all tubed media provided.
  4. Incubate plates and tubes at 35°C for 24 hours.

Results
Read and record your results across one line of the following table. Also record all results obtained by your neighbors with different isolates. Using table 24.3-1, identify the unknown organisms.


 
     
 
 
     




     
 
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