Estimation of cellulose
Cellulose, a major structural polysaccharide in plants, is the most abundant organic compound in nature, and is composed of glucose units joined together in the form of the repeating units of the disaccharide cellobiose with numerous cross linkages. It is also a major component in many of the farm wastes.
Cellulose undergoes acetolysis with acetic/nitric reagent forming acetylated cellodextrins wich gets dissolved and hydrolyzed to form glucose molecules on treatment with 67% H2SO4. This glucose molecule is dehydrated to form hydroxymethyl furfural which forms green colored product with anthrone and the color intensity is measured at 630nm.
» Acetic/Nitric Reagent: Mix 150ml of 80% acetic acid and 15mL of concentrated nitric acid.
» Anthrone: Dissolve 200mg anthrone in 100mL of ice-cold 95% sulphuric acid. Prepare fresh and chill for 2h before use.
» 67% sulphuric acid
1. Updegroff, D M (1969) Anal Biochem 32 420.
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