Estimation of reducing sugar by dinitrosalicylic acid method
For sugar estmation an alternative to Nelson-Somogyi method is the dinitrosalicylic acid method – simple, sensitive and adoptable during handling of a large number of samples at a time.
» Dinitrosalicylic Acid Reagent (DNS Reagent):
Dissolve by stirring 1g dinitrosalicylic acid, 200mg crystalline phenol and 50mg sodium sulphite in 100mL 1% NaOH. Store at 4°C. Since the reagent deteriorates due to sodium sulphite, if long storage is required, sodium sulphite may be added at the time of use.
» 40% Rochelle salt solution (Potassium sodium tartrate)
Calculate the amount of reducing sugars present in the sample using the standard graph.
1. Miller, G L (1972) Anal Chem 31 426.
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