The ABO system of blood group was introduced by Landsteiner in 1900. He
found 2 types of antigens present on the RBCs. They are antigen A and
antigen B. Similarly, there are 2 types of antibodies present in the plasma called antibody A and B.
Based on the presence or absence of antigen and antibodies, human blood
is classified into 4 groups A, B, AB, and O.
The A group contains antigen A and antibody B.
The B group contains antigen B and antibody A.
The AB group contains both antigens A and B and no antibodies.
The O group contains no antigen and both antibodies A and B.
The ABO blood group is inherited by a set of multiple alleles.
Presence of a particular factor is denoted by Rh factor discovered by Weiner
from the rabbits immunized with the blood of the Macaca rhesus monkey.
- Blood sample
- Applicator sticks
- Antisera A,B, and Rh
- Glass slide
- Spirit and disposable needles
- Clean a glass slide thoroughly.
- Place a drop of antiserum A on the left side, antiserum B on the right side,
and antiserum D at the center of the slide.
- Clean the tip of the index finger with cotton soaked in spirit.
- Prick the finger tip with the help of a sharp, sterilized disposable needle.
- Place 3 drops of blood near the antisera.
- Mix the blood and antiserum using application sticks.
Observe your experimental result.
Draw conclusions based on your result.