Determination of Blood Group and Rh Factor

Materials

• Blood sample
• Applicator sticks
• Antisera A,B, and Rh
• Glass slide
• Cotton
• Spirit and disposable needles

Procedure

1. Clean a glass slide thoroughly.
2. Place a drop of antiserum A on the left side, antiserum B on the right side, and antiserum D at the center of the slide.
3. Clean the tip of the index finger with cotton soaked in spirit.
4. Prick the finger tip with the help of a sharp, sterilized disposable needle.
5. Place 3 drops of blood near the antisera.
6. Mix the blood and antiserum using application sticks.

Result
Observe your experimental result.

Conclusion
Draw conclusions based on your result.