Elongation of polypeptide

In E. coli, after the formation of 70S-mRNA-f-met-tRNAfmet complex, elongation of polypeptide chain is brought about by the regular addition of amino acids. At 37°C about 15 amino acids per second are added to a growing polypeptide chain, so that in 20 seconds a protein with 300 amino acids can be synthesized. The following three steps are important in the elongation process.