Recovery of Cells Containing Recombinant Plasmid
Fig. 4.4. Colony hybridization technique.
Colonies complementary to nitrocellulose signal are recovered from the master plate and cultured in nutrient broth in order to get the sufficient bacterial clones containing recombinant plasmid (Fig. 4.4G). However, in some instances the foreign DNA is cleaved enzymatically from the vector molecules. Sometimes aim lies to achieve expression of gene within the bacterial cell if it encodes for valuable polypeptides.