Update image
Add image
Medical Microbiology / Cell Biology Methods
- General Techniques
- Setting up a Cell Culture Laboratory
- General Procedures for Cell Culture
- Counting Cells
- Cell Proliferation Assays:Improved Homogeneous Methods Used to Measure the Number of Cells in Culture
- Development of Serum-Free Media:Optimization of Nutrient Composition and Delivery Format
- Cell Line Authentication
- Detection of Microbial and Viral Contaminants in Cell Lines
- Culture of Specific Cell Types: Stem Cells
- Neural Crest Stem Cells
- Postnatal Skeletal Stem Cells: Methods for Isolation & Analysis of Bone Marrow Stromal Cells from Postnatal Murine & Human Marrow
- Establishment of Embryonic Stem Cell Lines from Adult Somatic Cells by Nuclear Transfer
- T-Cell Isolation and Propagation in vitro
- Generation of Human and Murine Dendritic Cells
- Culture of Specific Cell Types: Haemopoietic, Mesenchymal, and Epithelial
- Clonal Cultures in vitro for Haemopoietic Cells Using Semisolid Agar Medium
- Human Skeletal Myocytes
- Growing Madin-Darby Canine Kidney Cells for Studying Epithelial Cell Biology
- Cultivation and Retroviral Infection of Human Epidermal Keratinocytes
- Three-Dimensional Cultures of Normal and Malignant Human Breast Epithelial Cells to Achieve in vivo-like Architecture and Function
- Differentiation and Reprogramming of Somatic Cells
- Primary Culture of Drosophila Embryo Cells
- Laboratory Cultivation of Caenorhabditis elegans and Other Free-Living Nematodes
- Induction of Differentiation and Cellular Manipulation of Human Myeloid HL-60 Leukemia Cells
- Cultured for Neuronal PC12 Ce lls: A Model Function, Differentiation, and Survival
- Differentiation of Pancreatic Cells into Hepatocytes
- TERA2 and Its NTERA2 Subline: Pluripotent Human Embryonal Carcinoma Cells
- Embryonic Explants from Xenopus laevis as an Assay System to Study Differentiation of Multipotent Precursor Cells
- Electrofusion: Nuclear Reprogramming of Somatic Cells by Cell Hybridization with Pluripotential Stem Cells
- Reprogramming Somatic Nuclei and Cells with Cell Extracts
- Assays of Cell Transformation, Tumorigenesis, Invasion and Wound Healing
- Cellular Assays of Oncogene Transformation
- Assay of Tumorigenicity in Nude Mice
- Transfilter Cell Invasion Assays
- Endothelial Cell Invasion Assay
- Analysis of Tumor Cell Invasion in Organotypic Brain Slices Using Confocal Laser-Scanning Microscopy
- Angiogenesis Assays
- Three-Dimensional, Quantitative in vitro Assays of Wound Healing Behavior
- Growth and Purification of Viruses
- Growth of Semliki Forest Virus
- Design and Production of Human Immunodeficiency Virus-Derived Vectors
- Construction and Propagation of Human Adenovirus Vectors
- Production and Quality Control of High-Capacity Adenoviral Vectors
- Novel Approaches for Production of Recombinant Adeno-Associated Virus
- Production and Purification of Antibodies
- Production of Peptide Antibodies in Rabbits and Purification of Immunoglobulin
- Preparation of Monoclonal Antibodies
- Rapid Development of Monoclonal Antibodies Using Repetitive Immunizations, Multiple Sites
- Phage- Displayed Antibody Libraries
- Ribosome Display: In Vitro Selection of Protein-Protein Interactions
- Epitope Mapping by Mass Spectrometry
- Mapping and Characterization of Protein Epitopes Using the SPOT Method
- Determination of Antibody Specificity by Western Blotting
- Enzyme-Linked Immunosorbent Assay
- Radioiodination of Antibodies
- Isolation: Plasma Membrane, Organelles, and Cellular Structures
- Detergent-Resistant Membranes and the Use of Cholesterol Depletion
- Isolation and Subfractionation of Plasma Membranes to Purify Caveolae Separately from Lipid Rafts
- Immunoisolation of Organelles Using Magnetic Solid Supports
- Purification of Rat Liver Golgi Stacks
- Isolation of Rough and Smooth Membrane Domains of the Endoplasmic Reticulum from Rat Liver
- Purification of COPI Vesicles
- Purification of Clathrin-Coated Vesicles from Bovine Brain, Liver, and Adrenal Gland
- Isolation of Latex Bead- and Mycobacteria-Containing Phagosomes
- Isolation of Peroxisomes
- Isolation of Mitochondria from Mammalian Tissues and Cultured Cells
- Subcellular Fractionation Procedures and Metabolic Labeling Using [35S] Sulfate to Isolate Dense Core Secretory Granules from Neuroendocrine Cell Lines
- Preparation of Synaptic Vesicles from Mammalian Brain
- Prep aration of Proteasomes
- Preparation of Cilia from Human Airway Epithelial Cells
- Isolation of Nucleoli
- Purification of Intermediate-Containing Spliceosomes
- Isolation of Cajal Bodies
- Replicon Clusters: Labeling Strategies for the Analysis of Chromosome Architecture and Chromatin Dynamics
- Isolation of Chromosomes for Flow Analysis and Sorting
- Protein Purification
- Purification of Smooth Muscle Actin
- Purification of Nonmuscle Actin
- Purification of Skeletal Muscle Actin
- Permeabilized Epithelial Cells to Study Exocytic Membrane Transport
- Studying Exit and Surface Delivery of Post-Golgi Transport Intermediates Using in vitro and Live-Cell Microscopy-Based Approaches
- Endocytic and Exocytic Pathways
- Assays Measuring Membrane Transport in the Endocytic Pathway
- Microsome-B, ased Assay for Analysis of Endoplasmic Reticulum to Golgi Transport in Mammalian Cells
- Cotranslational Translocation of Proteins into Microsomes Derived from the Rough Endoplasmic Reticulum of Mammalian Cells
- Use of Permeabilised Mast Cells to Analyze Regulated Exocytosis
- Chromatin Assembly
- DNA Replication-Dependent Chromatin Assembly System
- Cygnets' Intracellular Guanosine 3',5'- Cyclic Monophosphate Sensing in Primary Cells Using Fluorescence Energy Transfer
- Ca2+ as a Second Messenger: New Reporters for Calcium (Cameleons and Camgaroos)
- Ratiometric Pericam
- Fluorescent Indicators for Imaging Protein Phosphorylation in Single Living Cells
- In Situ Electroporation of Radioactive Nucleotides: Assessment of Ras Activity or 32p Labeling of Cellular Proteins
- Signal Transduction Assays
- Dissecting Pathways; in Situ Electroporation for the Study of Signal Transduction and Gap Junctional Communication
- Detection of Protein-Protein Interactions in vivo Using Cyan and Yellow Fluorescent Proteins
- Tracking Individual Chromosomes with Integrated Arrays of lac op Sites and GFP-lac i Repressor: Analyzing Position and Dynamics of Chromosomal Loci in Saccharomyces cerevisiae
- Mechanical Stress in Single Cells
- Pedestal Formation by Pathogenic Escherichia coli: A Model System for Studying Signal Transduction to the Actin Cytoskeleton
- Listeria monocytogenes: Techniques to Analyze Bacterial Infection in vitro
- Measurement of Cellular Contractile Forces Using Patterned Elastomer
- Resources
- Digital Image Processing, Analysis, Storage, and Display
- Imaging Fluorescence Resonance Energy Transfer between Green Fluorescent Protein Variants in Live Cells
- Measurements of Endosomal pH in Live Cells by Dual-Excitation Fluorescence Imaging
- Genome-Wide Screening of Intracellular Protein Localization in Fission Yeast
- Large Scale Protein Localization in Yeast
- Lifting the Fog: Image Restoration by Deconvolution
- The State of the Art in Biological Image Analysis
- Publishing and Finding Images in the BioImage Database, an Image Database for Biologists
- Cytogenetics
- Basic Cytogenetic Techniques: Culturing, Slide Making, and G Banding
- A General and Reliable Method for Obtaining High-Yield Metaphasic Preparations from Adherent Cell Lines: Rapid Verification of Cell Chromosomal Content
- Mapping Cloned DNA on Metaphase Chromosomes Using Fluorescence in Situ Hybridization
- Human Genome Project Resources for Breakpoint Mapping
- Fine Mapping of Gene Ordering by Elongated Chromosome Methods
- Genomics
- Fluorescent Visualization of Genomic Structure and DNA Replication at the Single Molecule Level
- Genomic DNA Microarray for Comparative Genomic Hybridization
- Genotyping of Single Nucleotide Polymorphisms by Minisequencing Using Tag Arrays
- Single Nucleotide Polymorphism Analysis by Matrix-Assisted Laser Desorption/Ionization Time-of-F'light Mass Spectrometry
- Single Nucleotide Polymorphism Analysis by ZipCode-Tagged Microspheres
- Polymerase Chain Reaction-Based Amplification Method of Retaining the Quantitative Difference between Two Complex Genomes
