Update image
Add image
General Biotechnology / Genes & Genetic Engineering
Genes & Genetic Engineering
Section: Genes : Nature, Concept and Synthesis
- Chemical nature of DNA
- Chemical composition
- Nucleotides, nucleosides
- Polynucleotides
- Chargaff's rule of equivalence
- Physical nature of DNA
- Watson and Cricks model of DNA
- Circular and superhelical DNA
- Organization of DNA in eukaryotes
- Structure of RNA
- Gene concept
- Units of a gene
- Cistron
- Recon
- Mutan
- Split genes (introns)
- RNA splicing
- Ribozyme
- Evolution of split genes
- Overlapping gene
- Gene organization
- Gene expression
- Gene regulation
- Transcription
- The lacoperon (structural gene,operator gene, promoter gene and repressor gene)
- Artificial synthesis of genes
- Synthesis of a gene for yeast alanine tRNA
- Synthesis of a gene for bacteria ltyrosine tRNA
- Synthesis of a human leukocyte interferon gene
- Gene synthesis by using mRNA
- Gene machine
- The PCR
- Amplification of DNA (melting of target DNA, annealing of primers, primer extension)
- Application of PCR technology
Section: Tools of Genetic Engineering
- Enzymes
- Exonucleases
- Endonucleases
- Restriction endonucleases
- Nomenclature
- Example of some enzymes
- SI nuclease
- DNA ligases
- Alkaline phosphatase
- Reverse transcriptase
- DNA Polymerase
- Foreign DNA
- Cloning vectors
- Plasmids
- Bacteriophages
- Insertion vector
- Replacement vector
- Cosmids
- Phasmids
- cDNA Clone bank
- Gene bank (Genomic Library)
- Electrophoresis
Section: Techniques of Genetic Engineering
- Gene cloning in prokaryotes
- Isolation of DNA to be cloned
- Insertion of DNA fragment intovector
- Use of restriction Linkers
- Use of homopolymer tails
- Transfer of recombinant DNA intobacterial cells
- Selection of clones
- Colony hybridization techniques
- Invitrotranslation technique
- Immunologicaltests
- BlottingTechniques
- Recoveryof cells
- Expressionof cloned DNA
- Shine-Dalganosequence
- Expression vectors
- Genecloning in eukaryotes
- Plantcells
- Yeasts
- Filamentous fungi
- Agrobacterium plasmids
- Plant cell transformation
- Plant cell transformation byultrasonication
- Liposome mediated gene transfer
- Animal cell
- Animal viruses
- Electroporation
- Particle bombardment
- Microinjection
- Direct transformation
- Site directed mutagenesis
- Methods of mutagenesis
Section: Genetic Engineering for Human Welfare
- Cloned genes and production of chemicals
- Human peptide hormone genes
- Insulines
- Somatotropin
- Somatostatin
- b-endorphin
- Human interferon genes
- Genes for vaccines
- Vaccine for hepatitis-B virus
- Vaccines for Rabies virus
- Vaccines for poliovirus
- Vaccine for foot and mouth disease virus
- Vaccines for small pox virus
- Malaria vaccines
- DNA vaccines
- Genes associated with genetic diseases
- Phenylketonuria
- Urokinase
- Thalassaemia
- Hemophilia
- Enzyme engineering
- Commercial chemicals
- Prevention, diagnosis and cure ofdiseases
- Prevention of diseases
- Diagnosis of diseases
- Parasitic diseases
- Monoclonal antibodies
- Antenatal diagnosis
- Gene therapy
- Types of gene therapy
- Methods of gene therapy
- Success of gene therapy
- Potential of gene delivering system
- Future needs of gene therapy in India
- DNA profiling (fingerprinting)
- Methods of DNA profiling
- Application of DNA profiling
- Genetic databank
- Reuniting the lost children
- Solving disputed problems of parentage, identity of criminals, rapists, etc
- Immigrant dispute
- Hurdles of DNA profiling
- Animal and plant improvement
- Transgenic Farm Animals
- Crop Improvements
- Transgenic plants
- Nif gene transfer
- Phaseolin gene transfer
- Conversion of C3 plants to C4 plants
- Herbicide resistant plants
- Insect pest resistant plants
- Plant improvement through genetic transformation
- Crop Protection
- Use of antagonists
- Use of insecticides
- Abatement of pollution
Support our developers
