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Biotechnology Methods
- Biochemistry
- Carbohydrates
- Qualitative Tests
- Qualitative Analysis
- Estimate the Amount of Reducing Sugars
- Estimation of Reducing Sugar by Somogyi's Method
- Estimation of Sugar by Folin-Wu Method
- Estimation of Sugar by Hagedorn-Jenson Method
- Estimation of Reducing Sugars by the Dinitro Salicylic Acid (DNS) Method
- Determination of Blood Glucose by Hagedorn-Jenson Method
- Determining Blood Sugar by Nelson and Somogyi's Method
- Determination of Blood Glucose by the O-Toluidine Method
- Estimation of Protein by the Biuret Method
- Estimation of Protein by the FC-Method
- Protein Assay by Bradford Method
- Estimation of Protein by the Lowry Protein Assay
- Biuret Protein Assay
- Estimation of DNA by the Diphenylamine Method
- Estimation of RNA by the Orcinol Method
- Enzymology
- Enzymes
- Demonstrating the Presence of Catalase in Pig's Liver
- Determining the Optimum pH for Trypsin
- Extraction of Tyrosinase
- Preparation of Standard Curve
- Enzyme Concentration
- Effects of pH
- Effects of Temperature
- Computer Simulation of Enzyme Activity
- Kinetic Analysis
- Determination of Km and Vmax
- Addition of Enzyme Inhibitors
- Protein Concentration/Enzyme Activity
- Studying the Action and Activity of Amylase on Starch Digestion
- Determination of the Effect of pH on the Activity of Human Salivary a-Amylase
- Determining the Effect of Temperature on the Activity of Human Salivary a-Amylase
- Construction of the Maltose Calibration Curve
- Electrophoresis
- Electrophoresis
- Preparation of SDS-Polyacrylamide Gels
- Separation of Protein Standards: SDS-PAGE
- Coomassie Blue Staining of Protein Gels
- Silver Staining of Gels
- Documentation
- Western Blots
- Sodium Dodecyl Sulfate Poly-Acrylamide Gel Electrophoresis (SDS-PAGE)
- Preparation of Polyacrylamide Gels
- Agarose Gel Electrophoresis
- Microbiology
- Introduction
- The Microscopy
- The Bright Field Microscope
- Introduction to the Microscope and Comparison of Sizes and Shapes of Microorganisms
- Cell Size Measurements: Ocular and Stage Micrometers
- Measuring Depth
- Measuring Area
- Cell Count by Hemocytometer or Measuring Volume
- Measurement of Cell Organelles
- Use of Darkfield Illumination
- The Phase Contrast Microscope
- The Inverted Phase Microscope
- Aseptic Technique and Transfer of Microorganisms
- Control of Microorganisms by using Physical Agents
- Control of Microorganisms by using Disinfectants and Antiseptics
- Control of Microorganisms by using Antimicrobial Chemotherapy
- Isolation of Pure Cultures from a Mixed Population
- Bacterial Staining
- Direct Stain and Indirect Stain
- Gram Stain and Capsule Stain
- Endospore Staining and Bacterial Motility
- Enumeration of Microorganisms
- Biochemical Test for Identification of Bacteria
- Triple Sugar Iron Test
- Starch Hydrolysis Test (II Method)
- Gelatin Hydrolysis Test
- Catalase Test
- Oxidase Test
- IMVIC Test
- Extraction of Bacterial DNA
- Medically Significant Gram-Positive Cocci (GPC)
- Protozoans, Fungi, and Animal Parasites
- The Fungi, Part 1-The Yeasts
- Performance Objectives
- The Fungi, Part 2-The Molds
- Viruses: The Bacteriophages
- Serology, Part 1-Direct Serologic Testing
- Serology, Part 2-Indirect Serologic Testing
- Cell Biology and Genetics
- Cell Cycles
- Meiosis in Flower Buds of Allium Cepa-Acetocarmine Stain
- Meiosis in Grasshopper Testis (Poecilocerus Pictus)
- Mitosis in Onion Root Tip (Allium Cepa)
- Differential Staining of Blood
- Buccal Epithelial Smear and Barr Body
- Induction of Polyploidy
- Mounting of Genitalia in Drosophila Melanogaster
- Mounting of Genitalia in the Silk Moth Bombyx Mori
- Mounting of the Sex Comb in Drosophila Melanogaster
- Mounting of the Mouth Parts of the Mosquito
- Normal Human Karyotyping
- Karyotyping
- Black and White Film Development and Printing for Karyotype Analysis
- Study of Drumsticks in the Neutrophils of Females
- Study of the Malaria Parasite
- Vital Staining of DNA and RNA in Paramecium
- Sex-Linked Inheritance in Drosophila Melanogaster
- Preparation of Somatic Chromosomes from Rat Bone Marrow
- Chromosomal Aberrations
- Study of Phenocopy
- Study of Mendelian Traits
- Estimation of Number of Erythrocytes [RBC] in Human Blood
- Estimation of Number of Leucocytes (WBC) in Human Blood
- Culturing Techniques and Handling of Flies
- Life Cycle of the Mosquito (Culex Pipiens)
- Life Cycle of the Silkworm (Bombyx Mori)
- Vital Staining of Earthworm Ovary
- Culturing and Observation of Paramecium
- Culturing and Staining of E.coli (Gram’s Staining)
- Breeding Experiments in Drosophila Melanogaster
- Preparation of Salivary Gland Chromosomes
- Observation of Mutants in Drosophila Melanogaster
- ABO Blood Grouping and Rh Factor in Humans
- Determination of Blood Group and Rh Factor
- Demonstration of the Law of Independent Assortment
- Demonstration of Law of Segregation
- Molecular Biology
- The Central Dogma
- Protein Synthesis in Cell Free Systems
- Chromosomes
- Polytene Chromosomes of Dipterans
- Salivary Gland Preparation (Squash Technique)
- Extraction of Chromatin
- Chromatin Electrophoresis
- Extraction and Electrophoresis of Histones
- Karyotype Analysis
- In Situ Hybridization
- Culturing Peripheral Blood Lymphocytes
- Microslide Preparation of Metaphases for In-Situ Hybridization
- Staining Chromosomes (G-Banding)
- Nucleic Acids
- Extraction of DNA from Bovine Spleen
- Purification of DNA
- Characterization of DNA
- DNA-Dische Diphenylamine Determination
- Melting Point Determination
- CsCl-Density Separation of DNA
- Phenol Extraction of rRNA (Rat liver)
- Spectrophotometric Analysis of rRNA
- Determination of Amount of RNA by the Orcinol Method
- Sucrose Density Fractionation
- Nucleotide Composition of RNA
- Isolation of Genomic DNA—DNA Extraction Procedure
- Isolation of Genomic DNA from Bacterial Cells
- Preparation of Genomic DNA from Bacteria
- Extraction of Genomic DNA from Plant Source
- Extraction of DNA from Goat Liver
- Isolation of Cotton Genomic DNA from Leaf Tissue
- Arabidopsis Thaliana DNA Isolation
- Plant DNA Extraction
- Phenol/Chloroform Extraction of DNA
- Ethanol Precipitation of DNA
- Isolation of Mitochondrial DNA
- Isolation of Chloroplast DNA
- DNA Extraction of Rhizobium (CsCl Method)
- Isolation of Plasmids
- RNA Isolation
- Preparation of Vanadyl-Ribonucleoside Complexes that Inhibit Ribonuclease Activity
- RNA Extraction Method for Cotton
- Isolation of RNA from Bacteroids
- Isolation of RNA from Free-Living Rhizobia
- Estimation of DNA purity and Quantification
- Fungal DNA Isolation
- Methylene Blue DNA Staining
- Transformation
- Blotting Techniques—Southern, Northern, Western Blotting
- Preparing the Probe
- Southern Blotting (First Method)
- Southern Blotting (Second Method)
- Western Blotting
- Western Blot Analysis of Epitoped-tagged Proteins using the Chemifluorescent Detection Method for Alkaline Phosphatase-conjugated Antibodies
- Southern Blot
- Southern Analysis of Mouse Toe/Tail DNA
- Northern Blotting
- Restriction Digestion Methods—Restriction Enzyme Digests
- Restriction Digestion of Plasmid, Cosmid, and Phage DNAs
- Manual Method of Restriction Digestion of Human DNA
- Preparation of High-Molecular-Weight Human DNA Restriction Fragments in Agarose Plugs
- Restriction Enzyme Digestion of DNA
- Electroelution of DNA Fragments from Agarose into Dialysis Tubing
- Isolation of Restriction Fragments from Agarose Gels by Collection onto DEAE Cellulose
- Ligation of Insert DNA to Vector DNA
- PCR Methods (Polymerase Chain Reaction)
- Polymerase Chain Reaction
- DNA Amplification by the PCR Method
- Tissue Culture Techniques
- Tissue Culture Methods
- Plant Tissue Culture
- Plant Tissue Culture (Cont.)
- Many Dimensions of Plant Tissue Culture Research
- What is Plant Tissue Culture?
- Uses of Plant Tissue Culture
- Plant Tissue Culture demonstration by Using Somaclonal Variation to Select for Disease Resistance
- Demonstration of Tissue Culture for Teaching
- Preparation of Plant Tissue Culture Media
- Plant Tissue Culture Media
- Preparation of Protoplasts
- Protoplast Isolation, Culture, and Fusion
- Agrobacterium Culture and Agrobacterium — Mediated transformation
- Isolation of Chloroplasts from Spinach Leaves
- Preparation of Plant DNA using
- Suspension Culture and Production of Secondary Metabolites
- Protocols for Plant Tissue Culture
- Sterile Methods in Plant Tissue Culture
- Media for Plant Tissue Culture
- Safety in Plant Tissue Culture
- Preparation of Media for Animal Cell Culture
- Aseptic Technique
- Culture and Maintenance of Cell Lines
- Trypsinizing and Subculturing Cells from a Monolayer
- Cellular Biology Techniques
- In Vitro Methods
- Human Cell Culture Methods
