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  Section: Plant Nutrition » Other Beneficial Elements » Aluminum
 
 
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Aluminum Absorption and Transport within Plants

 
     
 
Content
Introduction
Aluminum-Accumulating Plants
Beneficial Effects of Aluminum in Plants
  Growth Stimulation
  Inhibition of Plant Pathogens
Aluminum Absorption and Transport within Plants
  Phytotoxic Species
  Absorption
  Aluminum Speciation in Symplasm
  Radial Transport
  Mucilage
Aluminum Toxicity Symptoms in Plants
  Short-Term Effects
    - Inhibition of Root Elongation
    - Disruption of Root Cap Processes
    - Callose Formation
    - Lignin Deposition
    - Decline in Cell Division
  Long-Term Effects
    - Suppressed Root and Shoot Biomass
    - Abnormal Root Morphology
    - Suppressed Nutrient Uptake and Translocation
    - Restricted Water Uptake and Transport
    - Suppressed Photosynthesis
    - Inhibition of Symbiosis with Rhizobia
Mechanisms of Aluminum Toxicity in Plants
  Cell Wall
    - Modification of Synthesis or Deposition of Polysaccharides
  Plasma Membrane
    - Binding to Phospholipids
    - Interference with Proteins Involved in Transport
      - H+ -ATPases
      - Potassium Channels
      - Calcium Channel
      - Magnesium Transporters
      - Nitrate Uptake
      - Iron Uptake
      - Water Channels
    - Signal Transduction
      - Interference with Phosphoinositide Signal Transduction
      - Transduction of Aluminum Signal
  Symplasm
    - Disruption of the Cytoskeleton
    - Disturbance of Calcium Homeostasis
    - Interaction with Phytohormones
      - Auxin
      - Cytokinin
    - Oxidative Stress
    - Binding to Internal Membranes in Chloroplasts
    - Binding to Nuclei
Genotypic Differences in Aluminum Response of Plants
  Screening Tests
  Genetics
Plant Mechanisms of Aluminum Avoidance or Tolerance
  Plant Mechanisms of Aluminum Avoidance
    - Avoidance Response of Roots
    - Organic Acid Release
    - Exudation of Phosphate
    - Exudation of Polypeptides
    - Exudation of Phenolics
    - Alkalinization of Rhizosphere
    - Binding to Mucilage
    - Binding to Cell Walls
    - Binding to External Face of Plasma Membrane
    - Interactions with Mycorrhizal Fungi
  Plant Mechanisms of Aluminum Tolerance
    - Complexation with Organic Acids
    - Complexation with Phenolics
    - Complexation with Silicon
    - Sequestration in Vacuole or in Other Organelles
    - Trapping of Aluminum in Cells
Aluminum in Soils
  Locations of Aluminum-Rich Soils
  Forms of Aluminum in Soils
  Detection or Diagnosis of Excess Aluminum in Soils
    - Extractable and Exchangeable Aluminum
    - Soil-Solution Aluminum
  Indicator Plants
Aluminum in Human and Animal Nutrition
  Aluminum as an Essential Nutrient
  Beneficial Effects of Aluminum
    - Beneficial Effects of Aluminum in Animal Agriculture
    - Beneficial Uses of Aluminum in Environmental Management and Water Treatment
  Toxicity of Aluminum to Animals and Humans
    - Toxicity to Wildlife
    - Toxicity to Agricultural Animals
      - Toxicity to Ruminants (Cattle and Sheep)
      - Toxicity to Poultry
    - Toxicity to Humans
      - Overview of Aluminum Metabolism
      - Overview of the Biochemical Mechanisms of Aluminum Toxicity
Aluminum Concentrations
  In Plant Tissues
    - Aluminum in Roots
    - Aluminum in Shoots
  Soil Analysis
References
 
Phytotoxic Species
The most phytotoxic form of aluminum is Al3+ (more correctly, Al(H2O)63+), which predominates in solutions below pH 4.5 (31-33) (Figure 16.1). Possibly, hydroxyl-aluminum (AlOH2+ and Al(OH)2+) ions are also phytotoxic, particularly to dicotyledonous plants (31,34). However, as pointed out by many researchers (35,36), these aluminum species are interrelated along with the pH variable, so it is difficult to rank their relative toxicity.


In contrast, Al-F, Al-SO4, and Al-P species are much less toxic or even nontoxic to plants (34,37). Barley (Hordeum vulgare L.) roots were unaffected by aluminum when 2.5 to 10 µM F- was added to nutrient solution containing up to 8 µM total soluble aluminum (37). Also using nutrient solution, Kinraide and Parker (38) positively demonstrated the nontoxic nature of Al-SO4 complexes (AlSO4+ and Al(SO4)2-) for wheat (Triticum aestivum L.) and red clover (Trifolium pratense L.). Soybean had longer root growth when increasing amounts of phosphorus were added to nutrient solutions having constant total aluminum concentrations (39).
 
FIGURE 16.1 Speciation of aluminum as affected by solution pH. (From R.B. Martin. Fe3+ and Al3+ hydrolysis equilibria. Cooperativity in Al3+ hydrolysis reactions. J. Inorg. Biochem. 44:141-147, 1991.)
 

Absorption
Since aluminum is a trivalent cation in its phytotoxic form in the external medium, it does not easily cross the plasma membrane. Akeson and Munns (40) calculated that the endocytosis of Al3+ could contribute to its absorption. Alternatively, it is possible that Al3+ could be absorbed through calcium channels (41) or nonspecific cation channels.


Our understanding of aluminum absorption across plant membranes has been limited by the complex speciation of Al, its binding to cell walls, lack of an affordable and available isotope, and lack of sensitive analytical techniques to measure low levels of aluminum in subcellular compartments (42). Aluminum absorption by excised roots of wheat, cabbage (Brassica oleracea L.), lettuce (Lactuca sativa L.), and kikuyu grass (Pennisetum clandestinum Hochst. ex Chiov.), and by cell suspensions of snapbean (Phaseolus vulgaris L.) followed biphasic kinetics (43-45). A rapid, nonlinear, nonmetabolic phase of uptake occurred during the first 20 to 30 min. This nonsaturable phase was thought to be accumulation in the apoplastic compartment due to polymerization or precipitation of aluminum or binding to exchange sites in cell walls (44). A linear, metabolic phase of uptake was superimposed over the nonlinear phase and thought to be accumulation in the symplasmic compartment (i.e., within the plasma membrane). Using the rare 26Al isotope and accelerator mass spectrometry on giant algal cells of Chara corallina Klein ex Willd., Taylor et al. (42) provided the first unequivocal evidence that aluminum rapidly crosses the plasma membrane into the symplasm. Accumulation of 26Al in the cell wall was nonsaturable during 3 h of aluminum exposure and accounted for most of aluminum uptake. Absorption of aluminum into the protoplasm occurred immediately but accounted for less than 0.05% of the total accumulation (42). Accumulation in the vacuole occurred after a 30-min lag period (42).

 


Aluminum Speciation in Symplasm
The pH of the cytoplasmic compartment generally ranges from 7.3 to 7.6 (5). Once aluminum enters the symplasm, the aluminate ion, Al(OH)4- or insoluble Al(OH)3 could form (Figure 16.1) (46). Alternatively, Al3+ could precipitate with phosphate as variscite, Al(OH)2H2PO4 (47). Based on higher stability constants, it is likely that Al3+ would be complexed by organic ligands, such as adenosine triphosphate (ATP) or citrate (47,48). Martin (47) hypothesized that based on their similar effective ionic radii and affinity for oxygen donor ligands, Al3+ would compete with Mg2+ rather than Ca2+ in metabolic processes.


Radial Transport
The main barrier to radial transport of aluminum across the root into the stele appears to be the endodermis. Rasmussen (49) used electron microprobe x-ray analysis to show little penetration of aluminum past the endodermis of corn (Zea mays L.) roots. Similarly, in Norway spruce (Picea abies H. Karst.) roots, a large aluminum concentration was detected outside the endodermis, but very low aluminum concentrations on the inner tangential wall (3,50). Using secondary-ion mass spectrometry, Lazof et al. (51) confirmed that the highest aluminum accumulation occurred at the root periphery of soybean root tips, with substantial aluminum in cortical cells, but very low aluminum in stellar tissues. Similar to calcium, aluminum is thought to bypass the endodermis, entering the xylem in maturing tissues where the endodermis is not fully suberized.




Mucilage
Aluminum must cross the root mucilage before it can penetrate to the root apical meristem. Mucilage is produced by the root cap and is a complex mixture of high-molecular-weight polysaccharides, a population of several thousand border cells, and an array of cell wall fragments (52). Archambault et al. (53) showed that aluminum binds tightly to wheat mucilage, with 25 to 35% of total aluminum remaining after citrate desorption.
 
     
 
 
     
     
 
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