- Polaroid camera (Fotodyne Foto/Phoresis I or equivalent) or 35-mm camera
equipped with macro lens
- Stained gel
- Photograph the gels.
- Use the photographs or negatives to measure the distance from the point
of protein application (or for 2 gel systems, the line separating the stacking
and separating gels) to the final location of the tracking dye near the
bottom of the gel.
- Measure the distance from the point of origin to the center of each band
appearing on the gel.
- Divide each of the values obtained in step 3 by that obtained in step 2 to
obtain the relative mobility (the Rf value) for each band.
- Using either the graph of Rf values and molecular weights from Exercise 2,
compute the molecular weights of each band.
Scan the negative with a densitometer and compute Rf values based on the
distances from the point of origin to the peak tracing for each protein band.
Integration of the area of each peak will yield quantitative data, as well as the