Safety in Plant Tissue Culture


Tissue Culture Techniques
  Tissue Culture Methods
  Plant Tissue Culture
  Plant Tissue Culture (Cont.)
  Many Dimensions of Plant Tissue Culture Research
  What is Plant Tissue Culture?
  Uses of Plant Tissue Culture
  Plant Tissue Culture demonstration by Using Somaclonal Variation to Select for Disease Resistance
  Demonstration of Tissue Culture for Teaching
  Preparation of Plant Tissue Culture Media
  Plant Tissue Culture Media
  Preparation of Protoplasts
  Protoplast Isolation, Culture, and Fusion
  Agrobacterium Culture and Agrobacterium — Mediated transformation
  Isolation of Chloroplasts from Spinach Leaves
  Preparation of Plant DNA using
  Suspension Culture and Production of Secondary Metabolites
  Protocols for Plant Tissue Culture
  Sterile Methods in Plant Tissue Culture
  Media for Plant Tissue Culture
  Safety in Plant Tissue Culture
  Preparation of Media for Animal Cell Culture
  Aseptic Technique
  Culture and Maintenance of Cell Lines
  Trypsinizing and Subculturing Cells from a Monolayer
  Cellular Biology Techniques
  In Vitro Methods
  Human Cell Culture Methods
  • Sterile tissue culture technique eliminates or almost eliminates the danger of loose seeds, pollen broadcast, vegetative escape, etc.
  • Bamboo is unlikely to cause gene flow due to long intervals between flowering.
  • Handling and growing cultures inside a locked and filtered plastic box isolates the organisms from infection and prevents escape.
  • Choosing clumping bamboos limits the possibilities of vegetative escape, even if planted in the ground.
Therefore, looking at standard practices for biosafety level 1-P:
  1. A locked apartment door and secure windows are more than enough access restriction, in this case.
  2. You can inform yourself of hazards and safeguards.
  3. Given an organism with low potential for gene flow, the procedures are more than adequate.
  4. No special containment is required other than keeping cultures inside the plastic glove box/growing chamber. If removed, they can be sealed inside a small plastic carrying box that is passed into the chamber using aseptic procedures.
  5. Records can be kept in a lab notebook.
  6. Supplies can be sterilized as they are passed into the glove box/growing chamber and sterilized again as they are passed out.
  7. All organisms can be inactivated by placing them inside a pressure cooker, which is sealed before removal from the chamber. The pressure cooker is not unsealed until after an adequate processing time.
  8. Maintenance in tissue culture and inactivation through pressure cooking provide adequate pest control.
  9. Since there will be no motile organisms, sterile tissue culture is adequate containment.
  10. Given the built-in safety of sterile tissue culture and the organisms selected no signage is needed.
  11. Working inside an air-tight glove box minimizes the creation of aerosols.
  12. Use of gloves eliminates the need for special clothing. Hand washing before and after procedures is adequate.
  13. Any accidents and cleanup procedures can be recorded in a lab notebook.