Singleton's method. W.R. Singleton also studied induced mutations. The pollen from plants dominant for several genes and growing in a field with radiation source were used to pollinate a rece'ssive stock growing in a field without radiation source. The pollen carrying mutations will give seeds, which will show recessive character in phenotype.
Mutations at unspecified loci
When a plant species is used where no marker stocks are available and the purpose is to study visible mutations in genome as a whole, the mutations are studied by segregation in M2 generation. The following steps will be involved :
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