Determination of glucose by glucose oxidase method
Glucose is a widely distributed simple sugar with an active aldehyde group. Estimation of glucose by glucose oxidase gives the true glucose concentration eliminating the interference by other reducing sugars.
» Glucose Oxidase Peroxidase Reagent:
» Dissolve 25mg O-dianisidine completely in 1mL of methanol. Add 49mL of 0.1M phosphate buffer (pH 6.5). then add 5mg of peroxidase and 5mg of glucose oxidase to the above prepared O-dianisidine solution.
» Standard: Dissolve 100mg glucose in 100mL water. Dilute 10mL of this stock to 100mL to obtain the working standard.
From the standard graph, calculate the amount of glucose present in the sample preparation.
1. Malik, C P and Singh, M B (1980) Plant Enzymology and Histoenzymology Kalyani Publishers New Delhi p 278.
2. Krishnaveni, S, Theymoli Balasubramanian and Sadasivam, S (1984) Food Chem 15 229.
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