Biological Nitrogen Fixation

Nitrogen is available in atmosphere in high amount in the form of gas. It is converted into combined form of organic compounds by some prokaryotic microorganisms through biological reactions. The phenomenon of fixation of atmospheric nitrogen/by biological means is known as 'diazotrophy' or 'biological nitrogen fixation' and these prokaryotes as 'diazotrophs' or 'nitrogen fixers'. The diazotrophs may be in free living or in symbiotic forms.

It is estimated that the free living microorganisms contribute about 1-3 kg N/ha/annum. During 1974, the total global nitrogen fixation was about 175 x 106 tonnes, of which 90 x 106 tonnes N was fixed in cultivated soil (Schlegel, 1986) and about 45 x 106 tonnes by rhizobial symbiosis.

In recent years, to meet the demand of nitrogen fertilizers, the number of chemical industries has been increased. These industries depend on fossil fuels; hence the cost of fertilizers is governed by that of fuels. In order to reduce dependence on chemical fertilizers, an alternative method is to be developed. Artificial inoculation of rice and other crop fields, with cyanobacteria has attracted much attention to promote rice production in developing countries, where fertilizers are expensive and in short supply. In this regard biological nitrogen fixation has been dealt with free living and symbiotic microorganisms.


» Non-Symbiotic N2 fixation
» Diazotrophy
» Ecology of diazotrophs
» Special features of diazotrophs
» Sites of N2 fixation
» Nitrogenase and reductants
» Presence of hydrogenase
» Self regulatory systems
» Mechanism of N2 fixation
» Symbiotic N2 fixation
» Establishment of symbiosis
» Host specificity and root hair curling
» Infection of root hairs
» Nodule development
» Nodule development and maintenance
» Factors affecting nodule development
» Mechanism of N2 fixation in root nodules
» Genetics of diazotrophs
» Nod genes
» Nif genes
» Nif gene cloning
» Hup genes