Ethylene, the ripening hormone is usually estimated in fruits during storage as well as for physiological studies. Ethylene level is also measured in infected plants.
The ethylene evolved is measured in a gas chromatograph based on the adsorption
Principle on activated silica gel or poropak.
» Conical Flasks or Cylinders with facility to seal with Rubber Gaskets
» Air-tight Syringes
» Ethylene Gas
» GLC - see, 'nitrogenase enzyme' for operating conditions.
The quantity of ethylene produced is expressed as ml ethylene per hour per kg material.
1. Depending upon the size and quantum of fruit sample, select suitable conical flasks or cylinders.
2. For studying rate of ripening select fruits without any macroscopic wounds and remove surface dirt.
1. Teitel, D C, Ahavoni, Y and Barkai-Golan, R (1989) J Hortic Sci 64 367.
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