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  Section: Biotechnology Methods » Molecular Biology
 
 
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Molecular Biology

 
     
 
Content
  Molecular Biology
    The Central Dogma
    Protein Synthesis in Cell Free Systems
    Chromosomes
    Polytene Chromosomes of Dipterans
    Salivary Gland Preparation (Squash Technique)
    Extraction of Chromatin
    Chromatin Electrophoresis
    Extraction and Electrophoresis of Histones
    Karyotype Analysis
    In Situ Hybridization
    Culturing Peripheral Blood Lymphocytes
    Microslide Preparation of Metaphases for In-Situ Hybridization
    Staining Chromosomes (G-Banding)
    Nucleic Acids
    Extraction of DNA from Bovine Spleen
    Purification of DNA
    Characterization of DNA
    DNA-Dische Diphenylamine Determination
    Melting Point Determination
    CsCl-Density Separation of DNA
    Phenol Extraction of rRNA (Rat liver)
   
    Spectrophotometric Analysis of rRNA
    Determination of Amount of RNA by the Orcinol Method
    Sucrose Density Fractionation
    Nucleotide Composition of RNA
    Isolation of Genomic DNA—DNA Extraction Procedure
    Isolation of Genomic DNA from Bacterial Cells
    Preparation of Genomic DNA from Bacteria
    Extraction of Genomic DNA from Plant Source
    Extraction of DNA from Goat Liver
    Isolation of Cotton Genomic DNA from Leaf Tissue
    Arabidopsis Thaliana DNA Isolation
    Plant DNA Extraction
    Phenol/Chloroform Extraction of DNA
    Ethanol Precipitation of DNA
    Isolation of Mitochondrial DNA
    Isolation of Chloroplast DNA
    DNA Extraction of Rhizobium (CsCl Method)
    Isolation of Plasmids
    RNA Isolation
    Preparation of Vanadyl-Ribonucleoside Complexes that Inhibit Ribonuclease Activity
    RNA Extraction Method for Cotton
    Isolation of RNA from Bacteroids
    Isolation of RNA from Free-Living Rhizobia
    Estimation of DNA purity and Quantification
    Fungal DNA Isolation
   
    Methylene Blue DNA Staining
    Transformation
    Blotting Techniques—Southern, Northern, Western Blotting
    Preparing the Probe
    Southern Blotting (First Method)
    Southern Blotting (Second Method)
    Western Blotting
    Western Blot Analysis of Epitoped-tagged Proteins using the Chemifluorescent Detection Method for Alkaline Phosphatase-conjugated Antibodies
    Southern Blot
    Southern Analysis of Mouse Toe/Tail DNA
    Northern Blotting
    Restriction Digestion Methods—Restriction Enzyme Digests
    Restriction Digestion of Plasmid, Cosmid, and Phage DNAs
    Manual Method of Restriction Digestion of Human DNA
    Preparation of High-Molecular-Weight Human DNA Restriction Fragments in Agarose Plugs
    Restriction Enzyme Digestion of DNA
    Electroelution of DNA Fragments from Agarose into Dialysis Tubing
    Isolation of Restriction Fragments from Agarose Gels by Collection onto DEAE Cellulose
    Ligation of Insert DNA to Vector DNA
    PCR Methods (Polymerase Chain Reaction)
    Polymerase Chain Reaction
    DNA Amplification by the PCR Method

 
     
 
 
     




     
 
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