Biotechnology of pepper
Peppers belong to the genus
Capsicum. They are found around the world in
various edible forms and they exhibit great variations in size, shape, flavour and
colour as well as plant habits. Five main species have been domesticated. (
Cfrutescens L.,
C. chinense Jacq. ,
C. baccatum L.,
C. pubescens R.& P., and
C.annuum L.) among which
C. annuum is the most widely cultivated. Two types of
cultivars can be distinguished: sweet and pungent. A horticultural classification
of peppershas been made by Smith
45 based on the size, shape, colour and taste
(sweet or pungent) of the fruit. The transfer of characters from wild species to
cultivated genotypes is hampered by interspecific incompatibility and/or hybrid
sterility. Only in a few cases have these barriers been overcome through embryo
culture and somatic hybridisation. A recent update on pepper
in vitro regeneration and transformation has been made by Steinitz
et al.,
46 and a
summary of genes of interest transferred into pepper is given in Table 10.2.
|
Table 10.2 A summary of gene transfer and corresponding agricultural traits into pepper and eggplant |
|
|
Plant species |
Transformation methods |
Gene utilised |
Agricultural traits |
Inheritance |
Field or greenhouse test |
Reference |
|
|
Capsicum annuum |
A.t. |
CMV coat
protein |
CMV resistance |
|
R1 |
51 |
|
|
A.t. |
CMV satellite RNA |
CMV resistance |
|
R1 |
59 |
|
|
A.t. |
Bar gene |
Herbicide resistance |
|
R1 |
60 |
|
|
Solanum melongena |
A.t. |
CryIIIB |
Insect resistance |
R1 |
No |
71 |
|
|
A.t. |
Synthetic
CryIIIA |
Insect resistance |
R1 |
F |
72 |
|
|
A.t. |
Mutagenised CryIIIB |
Insect resistance |
R2 |
F |
75, 76 |
|
|
A.t. |
Synthetic
CryIAb |
Insect resistance |
Not Tested |
No |
77 |
|
|
A.t. |
Cysteine
protease inhibitor |
Insect resistance |
|
No |
70 |
|
|
A.t. |
IaaM from Pseudomonas synringae |
Parthenocarpy |
R2 and more |
G |
79, 80 |
|