cDNA Clone Bank or cDNA Library
A typical eukaryotic cell contains between 10,000 to 30,000 different mRNA sequences. Williams (1981) has defined the cDNA clone bank as “a population of bacterial transformants, each containing a plasmid with a single cDNA insert, and with a sufficiently large number of individual transformants such that every mRNA molecule is represented at least once in the bacterial population”. A complete bank is that which contains in the order of 5,000 to 10,000 clones of the sequences amenable to detection.
(i) Preparation of cDNA clones is easy in many viruses whose genetic material is RNA, for example influenza virus, reovirus, etc.
(ii) Screening of cDNA is easy as it contains less number of cDNAs prepared from mRNA of the eukaryatic cells that may contain between 10,000 to 30,000 mRNA molecules. Hence, there is possibility of 10-30 thousand of cDNA fragments.
(iii) As the genes in prokaryotes do not contain introns (the intervening non-transcribed regions of DNA sequence which interrupt the transcripts of most of the genes); the cDNA clones possess an uninterrupted copy of mRNA molecules. It may also be attributed to the fact that the prokaryotes produce splicing enzymes for ligation process just to remove the sequences of introns. In contrast, in eukaryotes the introns spilt the transcribed. DNA into many fragments which are known as exons.
(iv) Following the hybridization process the nucleotide sequence of the gene interrupted by introns may be determined from a genomic clone by comparing with its mRNA transcripts therefore, a cloned cDNA is the most suitable probe for hybridization.