In Vitro Culture Techniques : The Biotechnological Principles

Root Culture
Generally excised root is cultured in liquid medium. It has several advantages over solid media. The techniques of root culture give certain important informations such as : (i) nutritional requirements, (ii) infection by Rhizobium and nodulation, and (iii) physiological activities, for example, production of alkaloids, nicotine, etc. (Dodds and Roberts, 1985). For the first time White (1934) reported the successful organ culture e.g. potentially unlimited growth of excised tomato roots. Subsequently, roots of several species of gymnosperms and angiosperms have been successfully cultured.
  Content
» Totipotency
» Historical background
» Requirements for cell and Tissue Cultures

» A tissues culture laboratory

» Nutrient media


» Inorganic chemicals


» Growth hormones


» Organic constitutents


» Vitamins


» Amino acids
» Culture of plant materials

» Explant culture

» Callus formation and its culture

» Organogenesis

» Root culture

» Shoot culture and micropropagation

» Cell culture


» Benefits from cell culture

» Somatic embryogenesis

» Somaclonal variation

» Protoplast culture


» Isolation


» Regeneration

» Protoplast fusion and somatic hybridization


» Fusion products


» Method of somatic hybridization

» Anther and pollen Culture


» Culturing techniques

» In vitro androgenesis (direct and indirect androgenesis)

» Mentor pollen technology

» Embryo culture

» Embryo rescue

» Protoplast fusion in fungi