The genital plate is located in the abdominal region of the male and female flies,
but in the males the genital plate is more prominent and used as a copulatory
pad. It is also called the epandrium. The genital plate is horseshoe-shaped, and
it is bent in the posterior region. It is divided into 2 parts, the heel and the toe.
Inside the arch, a pair of anal plates and a pair of primary claspersis are
present. On the primary claspers, 6–19 dark spines, or bristles are present,
which are very thick. Their number is species-specific. The main function of the
genital plate is to hold the female to transfer the sperm into her genital organ
- Male flies
- 1N HCl
- Cresosote solution
- Cavity slides
- Cover slips
- Remove the last abdominal segment of the male Drosophila melanogaster.
- Transfer the segment into 1N HCl taken in a cavity slide and allow it to
sit for 15 minutes.
- Blot out the HCl, use 2 or 3 drops of cresote solution, and allow it to sit
for about 20 minutes.
- Remove the pellicle organ and clean the genital plate.
- Transfer the genital plate onto a clean plain slide and mount the genital
plate with glycerine