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General Biotechnology / Genes & Genetic Engineering
Techniques of Genetic Engineering
- Gene cloning in prokaryotes
- Isolation of DNA to be cloned
- Insertion of DNA fragment intovector
- Use of restriction Linkers
- Use of homopolymer tails
- Transfer of recombinant DNA intobacterial cells
- Selection of clones
- Colony hybridization techniques
- Invitrotranslation technique
- Immunologicaltests
- BlottingTechniques
- Recoveryof cells
- Expressionof cloned DNA
- Shine-Dalganosequence
- Expression vectors
- Genecloning in eukaryotes
- Plantcells
- Yeasts
- Filamentous fungi
- Agrobacterium plasmids
- Plant cell transformation
- Plant cell transformation byultrasonication
- Liposome mediated gene transfer
- Animal cell
- Animal viruses
- Electroporation
- Particle bombardment
- Microinjection
- Direct transformation
- Site directed mutagenesis
- Methods of mutagenesis