Transfer of Recombinant DNA into Bacterial Cell
For the first time phage λ was used to transfer the foreign DNA into E. coli cell, therefore, it is often termed as transfection (a hybrid of transformation and infection). The efficiency of transformation is not high as it is influenced by bacterial strain and size of foreign DNA. It has been found that the efficiency of this process could generate about 105 transformants per mitrogram (Hg) of cloned circular plasmid (e.g. pBR322). It has not been possible to achieve efficiencies of over 108 transformants per g plasmid. If found that the efficiency of this process could generate about 105 transformants per microgram (mg) of cloned circular plasmid (e.g. pBR 322). It has not been possible to achieve efficiencies of over 108 transformants per g plasmid. If linear DNA is transformed it is almost completely insufficient in transformation.