You will note that for the determination of pH and temperature effects, we
established conditions that scanned a wide range of variables. To refine these
procedures, we would add more values, for example, temperatures from
30–40°C at steps of 1.0°C or 36–37°C with a step of 0.1°C. This is time-consuming.
To establish the principle, however, let’s shift to computer simulation of
enzyme activity. Computer simulations will also assist in the rapid accumulation
of data for kinetic analysis.
There are several excellent commercial programs available for enzyme kinetic
analysis, and use of these must be left to the discretion of the instructor. The
author has had excellent results with a rather old Basic program, ENZKIN,
which is still available from Conduit.
This is a simple, straightforward, and inexpensive program that is available
for Macs and mainframes. It can readily run on other machines with some
simple source code changes. Unfortunately, Conduit may not continue supplying
this program in the future.