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Biotechnology Methods / Molecular Biology
Molecular Biology
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- Molecular Biology
- The Central Dogma
- Protein Synthesis in Cell Free Systems
- Chromosomes
- Polytene Chromosomes of Dipterans
- Salivary Gland Preparation (Squash Technique)
- Extraction of Chromatin
- Chromatin Electrophoresis
- Extraction and Electrophoresis of Histones
- Karyotype Analysis
- In Situ Hybridization
- Culturing Peripheral Blood Lymphocytes
- Microslide Preparation of Metaphases for In-Situ Hybridization
- Staining Chromosomes (G-Banding)
- Nucleic Acids
- Extraction of DNA from Bovine Spleen
- Purification of DNA
- Characterization of DNA
- DNA-Dische Diphenylamine Determination
- Melting Point Determination
- CsCl-Density Separation of DNA
- Phenol Extraction of rRNA (Rat liver)
- Spectrophotometric Analysis of rRNA
- Determination of Amount of RNA by the Orcinol Method
- Sucrose Density Fractionation
- Nucleotide Composition of RNA
- Isolation of Genomic DNA—DNA Extraction Procedure
- Isolation of Genomic DNA from Bacterial Cells
- Preparation of Genomic DNA from Bacteria
- Extraction of Genomic DNA from Plant Source
- Extraction of DNA from Goat Liver
- Isolation of Cotton Genomic DNA from Leaf Tissue
- Arabidopsis Thaliana DNA Isolation
- Plant DNA Extraction
- Phenol/Chloroform Extraction of DNA
- Ethanol Precipitation of DNA
- Isolation of Mitochondrial DNA
- Isolation of Chloroplast DNA
- DNA Extraction of Rhizobium (CsCl Method)
- Isolation of Plasmids
- RNA Isolation
- Preparation of Vanadyl-Ribonucleoside Complexes that Inhibit Ribonuclease Activity
- RNA Extraction Method for Cotton
- Isolation of RNA from Bacteroids
- Isolation of RNA from Free-Living Rhizobia
- Estimation of DNA purity and Quantification
- Fungal DNA Isolation
- Methylene Blue DNA Staining
- Transformation
- Blotting Techniques—Southern, Northern, Western Blotting
- Preparing the Probe
- Southern Blotting (First Method)
- Southern Blotting (Second Method)
- Western Blotting
- Western Blot Analysis of Epitoped-tagged Proteins using the Chemifluorescent Detection Method for Alkaline Phosphatase-conjugated Antibodies
- Southern Blot
- Southern Analysis of Mouse Toe/Tail DNA
- Northern Blotting
- Restriction Digestion Methods—Restriction Enzyme Digests
- Restriction Digestion of Plasmid, Cosmid, and Phage DNAs
- Manual Method of Restriction Digestion of Human DNA
- Preparation of High-Molecular-Weight Human DNA Restriction Fragments in Agarose Plugs
- Restriction Enzyme Digestion of DNA
- Electroelution of DNA Fragments from Agarose into Dialysis Tubing
- Isolation of Restriction Fragments from Agarose Gels by Collection onto DEAE Cellulose
- Ligation of Insert DNA to Vector DNA
- PCR Methods (Polymerase Chain Reaction)
- Polymerase Chain Reaction
- DNA Amplification by the PCR Method
