Elongation of DNA chain

Content
Chemistry of the Gene 2.  Synthesis, Modification and Repair of DNA
DNA replication: general features 
Semi-conservative DNA replication in E. coli
Semi-conservative replication of chromosomes in eukaryotes
Semi-discontinuous DNA replication
Unidirectional and bidirectional DNA replication
RNA primers in DNA replication
Regulation of DNA replication by anti-sense RNA primer
Prokaryotic DNA polymerases
Eukaryotic DNA polymerases
Replicons for DNA replication
DNA replication in prokaryotes 
Experimental approaches for the study of DNA replication
Initiation of DNA replication
Elongation of DNA chain
Replication fork movement
Termination of DNA replication
DNA replication in eukaryotes 
DNA replication and cell cycle
Replication origins and initiation of DNA replication (cis and trans-acting elements)
Comparison of initiation of DNA replication with transcription initiation
Different steps involved in eukaryotic DNA replication
Synthesis of telomeric DNA by telomerase
Models of DNA replication
Replication fork model
Rolling circle model of DNA replication
Mitochondrial DNA replication and D-loops
RNA directed DNA synthesis (reverse transcription)
DNA modification and DNA restriction
DNA repair
Excision repair systems in E. coli
An SOS repair system in E. coli
DNA repair and genetic diseases in humans
Elongation of DNA chain
(replication elongation proteins)
As discussed above, initiation of DNA replication involves loading and activation of DnaB helicase. DnaB directs other necessary enzymes (particularly DnaG primase and DNA pol III HE) to the template and help in DNA elongation. These elongation proteins and the mechanism involved in elongation will be briefly described in this section.

DnaB protein. DnaB is the major helicase, although other helicases have been discovered. It unwinds the template in 5'-3' direction during oriC replication in vitro, at a rate of 700bp/sec. The direction of unwinding requires DnaB to be placed on lagging strand, ahead of the polymerase on the leading strand. It also activates primer synthesis by DnaG primase.
DnaG primase. The primase (DnaG) synthesizes a unique oligonucleotide primer on SSB coated single stranded phage G4 DNA. The sequence recognized by primase is over lOOnt (nt = nucleotides) long containing a secondary structure. Since primer synthesis requires activation by DnaB, the latter is often described as a 'mobile promoter' of primer synthesis.

DNA polymerase III holoenzyme. DNA pol III HE is the large multiprotein complex responsible for elongation of DNA replication in E. coli. It has at least 10 subunits, each with specific function (Table 26.3). The holoenzyme consists of a 'core polymerase' and its 'accessory factors', which are analogous to the components of phage T4 and eukaryotic enzymes. In the holoenzyme, β subunit is twice as abundant as the other subunits, and in the cell its abundance is 300 times that of the polymerase assembly. It has a 'sliding clamp' mode of DNA binding. It has similarity with T4 'gene 45' protein and human PCNA (see later).

genteic botany Biocyclopedia.com

Other proteins for DNA elongation. Other proteins involved in DNA elongation are (i) DNA gyrase (a topoisomerase), which removes the topological strain associated with DNA unwinding; (ii) SSB (single stranded DNA binding) protein, that binds tightly to single stranded DNA thus stabilizing the unwound DNA; (iii) RNase H, which removes RNA primers; (iv) DNA pol I, which is used for filling the gap created due to removal of RNA primers and (v) DNA ligase, which converts Okazaki fragments into a continuous strand.