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  Section: General Biotechnology / Microbial Biotechnology
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Single Cell Protein (SCP) and Mycoprotein


Substrates Used For Production of SCP

A variety of substrates are used for SCP production. However, availability of necessary substrates is of considerable biological and economic importance for the production of SCP. Algae which contain chlorophylls, do not require organic wastes. They use free energy from sunlight and carbondioxide from air, while bacteria (except photoautotrops) and fungi require organic wastes, as they do not contain chlorophylls (Roth, 1982).


The major components of substrates are the raw materials which contain sugars (sugarcane, sugarbeet and their processed products), starch (grains, tapioca, potato, and their by-products), lignocelluloses from woody plants and herbs having residues with nitrogen and phosphorous contents and other raw materials (whey and refuses from processed food). Organic wastes are also generated by certain industries and are rich in aromatic compounds or hydrocarbons (Bull et al, 1983). Recent price-increase in petroleum and refined petroleum products has made hydrocarbons and chemicals derived from them (such as methanol and ethanol) less attractive as raw materials for SCP production that renewable sources such as agricultural wastes or by-products. A detailed account of these wastes is given in Wastes as renewable source of energy.


Advantages of producing microbial protein

Microorganisms use as single cell protein (SCP)

Substrates used for the production of SCP

Nutritional values of SCP

Genetic improvements of microbial cells

Production of algal biomass


Factors affecting bio­mass production


Harvesting the algal biomass


Spirulina as SCP, cultivation and uses

Production of bacterial and actinomycetous biomass


Method of production


Factors affecting biomass production


Product recovery

Production of yeast biomass


Factors affecting growth of yeast


Recovery of yeast biomass

Production of fungal biomass (Other than Mushrooms)


Growth conditions


Organic wastes as substrates


Traditional fungal foods













Mushroom culture


Historical background


Present status of mushroom culture in India


Nutritional values


Cultivation methods



Obtaining pure culture 



Preparation of spawns



Formulation and preparation of composts



Spawning, spawn running and cropping


Control of pathogens and pests


Cultivation of paddy straw mushroom


Cultivation of white button mushroom


Cultivation of Dhingri (Pleurotus sajor-caju)


Recipes of mushroom


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