ASN-III Medium Composition
| Reagents | Per Liter Seawater |
|---|---|
| NaCl | 25 g |
| MgCl2 * 6H2O | 2 g |
| MgSO4 * 7H2O | 3.5 g |
| NaNO3 | 0.75 g |
| K2HPO4 * 3H2O | 0.02 g |
| KCl | 0.5 g |
| CaCl2 * 2H2O | 0.5 g |
| Citric acid (C6H8O7) | 0.003 g |
| Ammonium ferric citrate (C6H8O7 * nFe * nNH3) | 0.003 g |
| EDTANa2Mg | 0.0005 g |
| Na2Co3 | 0.02 g |
| Microelement stock solution | 1 ml |
| Microelement stock solution | |
| H3BO3 | 2.860 g |
| MnCl2 * 4H2O | 1.810 g |
| ZnSO4 * 7H2O | 0.222 g |
| Na2MoO4 * 2H2O | 0.390 g |
| CuSO4 * 5H2O | 0.079 g |
| Co(NO3)2 * 6H2O | 0.0494 g |
| ph = 7.5 | |
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