f/2 Medium Composition
| Reagents | Per Liter Seawater |
|---|---|
| NaNO3 | 0.075 g |
| NaH2PO4 * H2O | 0.005 g |
| Microelement stock solution | 1 ml |
| Vitamin solution | 1 ml |
| Microelement stock solution | |
| FeCl3 * 6H2O | 3.150 g |
| Na2EDTA | 4.160 g |
| MnCl2 * 4H2O | 0.180 g |
| CoCl2 * 6H2O | 0.010 g |
| CuSO4 * 5H2O | 0.010 g |
| ZnSO4 * H2O | 0.022 g |
| Na2MoO4 * 2H2O | 0.006 g |
| Vitamin stock solution | |
| Biotin (Vitamin H) | 0.5 mg |
| Thiamine HCl (Vitamin B1) | 100 mg |
| Cyanocobalamin (Vitamin B12) | 0.5 mg |
| ph = adjust to 8.0 with 1 M NAOH or HCl | |
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