K Medium Composition

ReagentsPer Liter Seawater
NaNO375 mg
Na2-glycerophosphate * 5H2O2.16 mg
NH4Cl2.67 mg
Tris-base (pH 7.2)121.1 mg
Microelement stock solution1 ml
Vitamin solution1 ml
Microelement stock solution
Fe Na EDTA4.3 g
Na2EDTA * 2H2O37.22 g
CuSO4 * 5H2O4.9 mg
MnCl2 * 4H2O178.2 mg
ZnSO4 * 7H2O22 mg
Na2MoO4 * 2H2O6.3 g
H2SeO31.29 µg
FeCl3 * 6H2O3.15 g
CoCl2 * 6H2O11.9 mg
Vitamin stock solution
Biotin (Vitamin H)0.1 mg
Thiamine HCl (Vitamin B1)200 mg
Cyanocobalamin (Vitamin B12)1 mg
ph = 7.2

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