Walne’s Medium Composition
| Reagents | Per Liter Seawater |
|---|---|
| Solution A | 1.0 ml |
| Solution C | 0.1 ml |
| Solution D (to add for diatoms) | 2.0 ml |
| Solution A | |
| FeCl3 * 6H2O | 1.3 g |
| MnCl2 * 4H2O | 0.4 g |
| H3BO3 | 33.6 g |
| Na2EDTA | 45.0 g |
| NaH2PO4 * 2H2O | 20.0 g |
| NaNO3 | 100.0 g |
| Solution B | 1 ml |
| Solution B per 100ml | |
| ZnCl2 | 2.1 g |
| CoCl2 * 6H2O | 2.0 g |
| (NH4)6Mo7O24 * 4H2O | 0.9 g |
| CuSO4 * 5H2O | 2.0 g |
| Concentrated HCl | 10 ml |
| Solution C per 200ml | |
| Thiamine HCl (Vitamin B1) | 0.2 g |
| Cyanocobalamin (Vitamin B12) | 10 mg |
| Solution D (per liter) | |
| Na2SiO3 * 5H2O | 40.0 g |
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