DY-III Medium Composition
| Reagents | Per Liter |
|---|---|
| MgSO4 * 7H2O | 50 mg |
| KCl | 3 mg |
| NH4Cl | 2.68 mg |
| NaNO3 | 20 mg |
| H3BO3 | 0.8 mg |
| Na2EDTA * 2H2O | 8 mg |
| Na2SiO3 * 9H2O | 14 mg |
| FeCl3 * 6H2O | 1 mg |
| CaCl2 | 75 mg |
| MES buffer | 200 mg |
| Microelement stock solution | 1 ml |
| Vitamin solution | 1 ml |
| Microelement stock solution | |
| MnCl2 * 4H2O | 200 mg |
| ZnSO4 * 7H2O | 40 mg |
| CoCl2 * 6H2O | 8 mg |
| Na2MoO4 * 6H2O | 20 mg |
| Na3VO4 * nH2O | 2 mg |
| H2SeO3 | 2 mg |
| Vitamin stock solution | |
| Biotin (Vitamin H) | 0.5 µg |
| Thiamine HCl (Vitamin B1) | 100 mg |
| Cyanocobalamin (Vitamin B12) | 0.5 mg |
| ph = 6.7 | |
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