Performance Objectives
- Define mycology and mycosis.
- Describe 3 ways fungi may be beneficial to humans and 3 ways they may be harmful.
Discussion
- Describe the typical appearance of a yeast and its usual mode of reproduction.
- Describe yeasts in terms of their oxygen requirements.
- Explain 2 ways the yeast Saccharomyces is beneficial to humans.
- Name 2 yeasts that commonly infect humans.
- Name 4 common forms of candidiasis.
- Describe 2 conditions that may enable Candida to cause severe opportunistic systemic infections.
- Describe pseudohyphae, blastospores, and chlamydospores.
- Explain the usefulness of saboraud dextrose agar, mycosel agar, and rice extract agar.
- Describe how Cryptococcus neoformans is transmitted to humans, where in the body it normally infects, and possible complications.
- Name the primary method of identifying Cryptococcus neoformans.
- Name which disease is caused by Pneumocystis carinii and indicate several predisposing conditions a person normally has before they contract the disease.
Results
- Describe the appearance of Saccharomyces cerevisiae and Candida albicans on saboraud dextrose agar and mycosel agar.
- When given a plate of Mycosel agar showing yeast-like growth and a plate of rice extract agar showing pseudohyphae, blastospores, and chlamydospores, identify the organism as Candida albicans.
- Recognize the following observed microscopically:
- Saccharomyces cerevisiae and Candida albicans as yeasts in a direct stain preparation.
- A positive specimen for thrush by the presence of budding Candida albicans.
- Cryptococcus neoformans in an India ink preparation.
- A cyst of Pneumocystis carinii in lung tissue.