Inverted phase microscope. Monolayer cultured cells or a suspension of protozoa
in a plastic disposable petri plate.
- The components in an inverted phase microscope are inverted. The phase
condenser may be an annular ring or a phase slider.
- Place either a culture flask containing a monolayer of cells or a plastic
petri plate containing a suspension of protozoa on the stage of the
- Observe the culture with normal bright field and with phase contrast. Note
any movements of the cells, and, over a period of time, note any changes
in the nuclear material of the monolayer cells.
- Draw and label your observations.