Among eukaryotes, DNA cloning has been done in yeast, mouse and to some extent even in some higher plant species. In yeast, a plasmid called 2μ DNA (63bp) is found, which is an appropriate cloning vehicle. An efficient transformation method is also available, which involves protoplast production and PEG (polyethylene glycol) directed introduction of DNA. In animal cells like mouse cells, special animal viruses were used as cloning vehicles. Simian virus 40 (SV40) is one such virus, in which globin gene could be integrated. This gene integrated in SV40 could be transcribed and translated in mouse kidney cells.
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