Besides cleavage, modification in the form of methylation is also brought about by some enzymes called modification enzymes (sometimes also called methylases).
This methylation distinguishes genes in different states of functioning. There are alsp enzymes which perform the function of restriction (cleavage) and modification (methylation). Based on these attributes restriction enzymes have been grouped into two classes : (a) type II restriction enzyme
systems (e.g. ZscoRI), which have separate enzymes for modification and restriction and (b) type
)and type III
15)enzyme systems, in which same enzyme possesses both activities (bifunctional), although the restriction and modification sites differ in position. (For more details, consult Chemistry of the Gene 2. Synthesis, Modification and Repair of DNA
Of the above two classes of restriction enzymes, type II enzymes are most important for cloning purposes. Some enzymes introduce staggered cuts, others generate blunt ends. Enzymes with 4bp target sites are used when frequent cuts are desired and those with 8bp are used when rare cuts are desired to get long DNA segments. Otherwise majority of enzymes used have 6 bp target sites. Some of them can cleave both methylated as well as unmethylated targets, but majority of them cleave only unmethylated targets. Details of some of these enzymes are listed in Table 39.1.