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Genetics / Genetic Engineering and Biotechnology / Recombinant DNA and PCR

Transposons as vectors

Transposons as vectors
Transposons of higher plants (e.g. Ds, Ac or Mul of maize) and transposons of Drosophila (P elements) are also used as vectors.

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More in this section

  • Recombinant DNA and PCR
  • Restriction enzymes in cloning
  • Techniques used in recombinant DNA
  • Cloning vectors for recombinant DNA
  • Plasmids as vectors
  • Bacteriophages as vectors
  • Plant and animal viruses as vectors
  • Transposons as vectors
  • Artificial chromosome vectors for cloning large DNA segments
  • Construction of chimeric DNA
  • Palindromes and staggered cleavage
  • Adding poly dA at the 3' ends of the vector and poly dT at the 3' ends of DNA clone
  • Blunt end ligation by T4 DNA ligase
  • Cloning in bacteria and eukaryotes
  • Cloning in bacteria
  • Cloning in eukaryotes
  • Molecular probes
  • Labelling of probes
  • Applications of molecular probes
  • Construction and screening of genomic and cDNA libraries
  • Gene amplification : PCR and its applications
  • cDNA library from mRNA
  • Colony (or plaque) hybridization for screening of libraries
  • Gene Amplification : PCR and Its Applications
  • The basic polymerase chain reaction (PCR)
  • Different schemes of PCR

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