Construction of chimeric DNA

Construction of Chimeric DNA
Chimeric DNA molecules are produced by inserting a foreign DNA segment into a DNA molecule of a vector. These chimeric DNA molecules are the means of cloning genes or other small DNA sequences. The construction of chimeric DNA, as we know now, involves joining of the broken ends of the vector with the two ends of the sequence to be cloned. There are atleast three methods available for the construction of this chimeric DNA.