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  Section: Biotechnology Methods » Cell Biology and Genetics
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ABO Blood Grouping and Rh Factor in Humans

Cell Biology and Genetics
  Cell Cycles
  Meiosis in Flower Buds of Allium Cepa-Acetocarmine Stain
  Meiosis in Grasshopper Testis (Poecilocerus Pictus)
  Mitosis in Onion Root Tip (Allium Cepa)
  Differential Staining of Blood
  Buccal Epithelial Smear and Barr Body
  Vital Staining of DNA and RNA in Paramecium
  Induction of Polyploidy
  Mounting of Genitalia in Drosophila Melanogaster
  Mounting of Genitalia in the Silk Moth Bombyx Mori
  Mounting of the Sex Comb in Drosophila Melanogaster
  Mounting of the Mouth Parts of the Mosquito
  Normal Human Karyotyping
  Black and White Film Development and Printing for Karyotype Analysis
  Study of Drumsticks in the Neutrophils of Females
  Study of the Malaria Parasite
  Vital Staining of DNA and RNA in Paramecium
  Sex-Linked Inheritance in Drosophila Melanogaster
  Preparation of Somatic Chromosomes from Rat Bone Marrow
  Chromosomal Aberrations
  Study of Phenocopy
  Study of Mendelian Traits
  Estimation of Number of Erythrocytes [RBC] in Human Blood
  Estimation of Number of Leucocytes (WBC) in Human Blood
  Culturing Techniques and Handling of Flies
  Life Cycle of the Mosquito (Culex Pipiens)
  Life Cycle of the Silkworm (Bombyx Mori)
  Vital Staining of Earthworm Ovary
  Culturing and Observation of Paramecium
  Culturing and Staining of E.coli (Gram’s Staining)
  Breeding Experiments in Drosophila Melanogaster
  Preparation of Salivary Gland Chromosomes
  Observation of Mutants in Drosophila Melanogaster
  ABO Blood Grouping and Rh Factor in Humans
  Determination of Blood Group and Rh Factor
  Demonstration of the Law of Independent Assortment
  Demonstration of Law of Segregation

The ABO system of blood group was introduced by Landsteiner in 1900. He found 2 types of antigens present on the RBCs. They are antigen A and antigen B. Similarly, there are 2 types of antibodies present in the plasma called antibody A and B. Based on the presence or absence of antigen and antibodies, human blood is classified into 4 groups A, B, AB, and O. The A group contains antigen A and antibody B. The B group contains antigen B and antibody A. The AB group contains both antigens A and B and no antibodies. The O group contains no antigen and both antibodies A and B. The ABO blood group is inherited by a set of multiple alleles. Presence of a particular factor is denoted by Rh factor discovered by Weiner from the rabbits immunized with the blood of the Macaca rhesus monkey.

  • Blood sample
  • Applicator sticks
  • Antisera A,B, and Rh
  • Glass slide
  • Cotton
  • Spirit and disposable needles
  1. Clean a glass slide thoroughly.
  2. Place a drop of antiserum A on the left side, antiserum B on the right side,
    and antiserum D at the center of the slide.
  3. Clean the tip of the index finger with cotton soaked in spirit.
  4. Prick the finger tip with the help of a sharp, sterilized disposable needle.
  5. Place 3 drops of blood near the antisera.
  6. Mix the blood and antiserum using application sticks.
Observe your experimental result.

Draw conclusions based on your result.


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